【作者】 谢迎；

【导师】 刘斌；

【作者基本信息】 兰州大学 ， 口腔医学， 2009， 硕士

【摘要】 目的:研究骨诱导材料脱钙牙基质的形貌、结构和骨诱导活性,为脱钙牙基质临床应用提供实验和理论依据。方法:1.以标准羟基磷灰石为对照,采用场发射扫描电镜,付立叶红外光谱仪,X一射线衍射光谱仪对脱钙牙基质的大小、形貌、组成及晶相结构进行观测和分析。2.以标准羟基磷灰石粉末为对照组,将脱钙牙基质与MC-3T3成骨细胞在体外复合培养,采用MTT法和ALP检测法检测脱钙牙基质对成骨细胞的增殖及碱性磷酸酶活性的影响。结果:1.脱钙牙基质颗粒呈圆棒状,大小均匀,成分主要为羟基磷灰石,并含有CaPO₃（OH） 2H₂O和含水的Ca₃（PO₄）₂等磷酸盐成分。2.脱钙牙基质能够促进成骨细胞的增殖及提高碱性磷酸酶的活性。结论:脱钙牙基质具有羟基磷灰石的主要物理特征,具有良好的生物相容性和骨诱导活性。更多还原

【Abstract】 Objective:Morphology,structure and bone induction activity of demineralized dental matrix were studied to provide experimental and theoretical base for the clinical application of the demineralized dentin matrix（DDM）.Methods:1.With standard hydroxyapatite as control,the morphology, structure and bone induction activity of the demineralized dental matrix were characterized by scanning electron microscopy,Fourier transform infrared spectroscopy and X-ray grating spectrum.2.MC-3T3 osteoblasts were cultured with demineralized dental matrix in vitro with standard hydroxyapatite as control.The influence of demineralized dental matrix on proliferation and alkaline phosphatase activity in osteoblasts were evaluated by MTT and ALP methods.Results:1.Demineralized dental matrix consists mainly of hydroxyapatite, and contains other phosphates such as CaPO₃（OH）·2H₂O and Ca₃（PO₄） ₂·XH₂O.Its granules were rod-shape and same in size.2.Demineralized dental matrix can promote proliferation and alkaline phosphatase activity of osteoblasts.Conclusion:Demineralized dental matrix has the chiefly characterization of hydroxyapatite,it is an ideal filling material with great osteoconduction and biocompatibility.更多还原