糖尿康丸主要药效学研究及其降血糖作用机制初探

【作者】 高爽；

【导师】 程嘉艺；

【作者基本信息】 辽宁中医药大学 ， 药理学， 2009， 硕士

【摘要】 目的:观察糖尿康丸的降血糖作用,初步了解糖尿康丸的急性毒性,并初步探讨糖尿康丸降血糖作用的机制。材料与方法:观察糖尿康丸对四氧嘧啶所致实验性糖尿病模型小鼠以及葡萄糖和肾上腺素引起的高血糖小鼠的血糖的影响。用放射免疫测定法测定糖尿康丸对四氧嘧啶所致实验性糖尿病模型小鼠血清胰岛素含量的影响。用蒽酮显色法测定糖尿康丸对四氧嘧啶所致实验性糖尿病模型小鼠肝糖原含量的影响。结果:1.急性毒性试验:糖尿康丸经口给药的最大给药量为41.93g生药/kg,按kg体重换算相当于临床用药量(17.28g生药/人/日=0.247g生药/kg)的170倍,小鼠未见异常表现,并无一例死亡。2.药效学实验:糖尿康丸中剂量组(9.0g生药/kg)可明显降低四氧嘧啶所致实验性糖尿病模型小鼠7天血糖含量,低剂量组(4.5g生药/kg)、中剂量组(9.0g生药/kg)和高剂量组(18.0g生药/kg)可明显降低四氧嘧啶所致实验性糖尿病模型小鼠14天血糖含量;糖尿康丸低剂量组(4.5g生药/kg)、中剂量组(9.0g生药/kg)和高剂量组(18.0g生药/kg)可明显降低葡萄糖性高血糖小鼠血糖含量;糖尿康丸中剂量组(9.0g生药/kg)、高剂量组(18.0g生药/kg)可明显降低肾上腺素性高血糖小鼠在注射肾上腺素后0.5小时血糖含量;低剂量组(4.5g生药/kg)、中剂量组(9.0g生药/kg)和高剂量组(18.0g生药/kg)可明显降低肾上腺素性高血糖小鼠在注射肾上腺素后1小时的血糖含量;糖尿康丸低剂量组(4.5g生药/kg)、中剂量组(9.0g生药/kg)和高剂量组(18.0g生药/kg)可明显增加四氧嘧啶所致实验性糖尿病模型小鼠血清胰岛素的含量;糖尿康丸低剂量组(4.5g生药/kg)、中剂量组(9.0g生药/kg)和高剂量组(18.0g生药/kg)可明显增加四氧嘧啶所致实验性糖尿病模型小鼠肝糖原的含量。结论:1.急性毒性试验:糖尿康丸口服给药较安全。2.药效学实验:糖尿康丸能明显降低四氧嘧啶、葡萄糖、肾上腺素所造成的高血糖小鼠的血糖含量,说明其具有显著的降低血糖、改善正常小鼠对葡萄糖的耐受能力作用;糖尿康丸可能通过减轻四氧嘧啶所致的胰岛β细胞损伤,并修复损伤的胰岛β细胞而增加四氧嘧啶模型小鼠血清胰岛素含量;可能通过抑制糖原分解,促进糖原合成而增加四氧嘧啶模型小鼠的肝糖原含量。更多还原

【Abstract】 Purpose: To observe the hypoglycemic effect of Tangniaokang pills, come tounderstand the acute toxicity of Tangniaokang pills preliminarily, and explore the hypoglycemic mechanism that Tangniaokang pills effect.Materials and methods : To observe the effect of blood sugar whichTangniaokang pills make on the experimental diabetes model mice and glucose and adrenaline-induced hyperglycemia in mice that were caused by alloxan. Using the radioimmunoassay to evaluate the effect which Tangniaokang pills make on the content of the experimental diabetes model mice serum insulin that were caused by alloxan. With anthrone color mensuration to evaluate the effect which Tangniaokang pills make on the content of the experimental diabetic mice liver glycogen.Results:1. Acute toxicity test: The largest drug delivery content of Tangniaokang pill oral administration is 41. 93g pharmacognostic / kg, according to the clinical equivalent conversion kg dosage (17. 28g pharmacognostic / person / day = 0. 247g pharmacognostic / kg) of 170 times. Mice show no abnormal and no dead case.2. Pharmacodynamics experiment: Tangniaokang pills Middle dose group (9.0g pharmacognostic /kg) can reduce 7days blood sugar content of experimental diabetic mice which were caused by alloxan significantly, Tangniaokang pills Low-dose group (4.5g pharmacognostic /kg )、Middle dose group ( 9.0g pharmacognostic/kg) and High-dose group (18.0g pharmacognostic/kg) can reduce 14days blood sugar content of experimental diabetic mice which were caused by alloxan significantly; Tangniaokang pills Low-dose group (4. 5g pharmacognostic /kg)、Middle dose group (9.0g pharmacognostic/kg) and High-dose group (18.0g pharmacognostic /kg) can reduce the blood sugar content of hyperglycemia experimental diabetic mice; Middle dose group (9.0g pharmacognostic /kg) and High-dose group (18.0g pharmacognostic /kg) can reduce the blood sugar content of the adrenaline-induced hyperglycemia mice which were injected adrenaline 0. 5 hours significantly; Tangniaokang pills Low-dose group (4. 5g pharmacognostic /kg)、Middle dose group (9.0g pharmacognostic/kg) and High-dose group (18.0g pharmacognostic /kg ) can reduce the blood sugar content of the adrenaline-induced hyperglycemia mice which were injected adrenaline 1 hours later significantly ; Tangniaokang pills Low-dose group (4.5g pharmacognostic /kg)、Middle dose group (9.0g pharmacognostic/kg) and High-dose group (18.0g pharmacognostic /kg ) can increase the serum insulin levels of the alloxan-induced experimental diabetic mice significantly; Tangniaokang pills Low-dose group (4.5g pharmacognostic /kg )、Middle dose group (9.0g pharmacognostic /kg)and High-dose group(18.0g pharmacognostic /kg)can increase the liver glycogen content of the alloxan-induced experimental diabetic mice significantly.Conclusions:1. Acute toxicity test: Tangniaokang pills are safe for oral drug delivery.2. Pharmacodynamics: Tangniaokang pills can significantly reduce the blood sugar levels of experimental alloxan-induced diabetic mice, can significantly reduce the blood sugar levels of the experimental mice which have reduction in blood sugar, improving the glucose tolerance of the normal mice; probably increase the serum insulin content of the alloxan-induced experimental diabetic mice by reducing alloxan-induced isletβcell damage, repairing damage of isletβcell; probably increase the liver glycogen content of the alloxan-induced experimental diabetic mice by inhibiting glycogenolysis, promoting the glycogen synthase.更多还原