【作者】 许良飞；

【导师】 石大兴； 李俊强；

【作者基本信息】 四川农业大学 ， 园林植物与观赏园艺， 2009， 硕士

【摘要】 本研究以四季海棠（Begonia Semperflorens）和丽格海棠（Begonia x hiemalis Fostsch）的叶片为试验材料,以器官发生途径对两种秋海棠进行无性繁殖体系的建立,并对它们最适宜的组织培养条件进行了对比。1.消毒时间:试验研究了0.1%的HgCl₂对两种秋海棠叶片的最佳消毒时间,结果表明:四季海棠叶片采用0.1%的HgCl₂灭菌5min较为适宜;丽格海棠叶片灭菌6min是较为适宜的。2.最佳取材时间:试验分别于1月、3月、5月、6月、10月、11月、12月对两种秋海棠进行取材接种,研究了它们的最佳取材时间,得出11月至12月上旬是四季海棠接种的最佳时期:丽格海棠在3月份取材最佳,其次是10月份。3.初代培养:试验采用三因素三水平的正交试验设计研究了两种秋海棠的最佳启动激素配方,四季海棠的最佳启动培养基是:1/2MS+6-BA0.5mg/L+IBA0.8mg/L;最有利于丽格海棠启动的诱导培养基配方是:1/2MS+6-BA1.0mg/L+NAA0.4mg/L。4.继代培养:采用完全试验研究了两个组合6-BA与NAA,6-BA与IBA对两种秋海棠的增殖影响,研究表明:四季海棠最佳增殖培养基为MS+6-BA0.5mg/L+NAA0.3mg/L;丽格海棠以MS+6-BA1.0mg/L+IBA0.5mg/L增殖效果较好。5.生根壮苗培养:试验在液体生根培养研究出四季海棠和丽格海棠最适宜的基本培养基类型、蔗糖浓度、生长素种类的基础上,又进行了固体培养详细研究了各激素的最适浓度,结果表明,四季海棠以液体培养基+MS+GA₃0.5mg/L+NAA0.5mg/L+蔗糖30g/L;丽格海棠的最佳生根壮苗培养基为液体培养基+MS+IBA1.0mg/L+蔗糖20g/L。6.移栽与炼苗:试验将具有不同类型根（由不同类型培养基培养所得）的小苗用三种浇水法——传统浇水法、浸水法、隔天种植法来培养,对比选出最佳的炼苗方法,得出四季海棠最佳的炼苗方案为液体培养所得的小苗用隔天种植法培养;丽格海棠最适炼苗方案为不加AC固体培养所得小苗用传统浇水法培养。更多还原

【Abstract】 The adexual propagation system of Begonia Semperflorens and Begonia x hiemalis Fostsch were established in this research,with material of leaf blade and way of organogenesis,moreover,the most suitable tissue culture condition of two kinds of Begonia plants was compared.1.Disinfection Time:The most appropriate disinfect time processed by HgCl₂ of 0.1% was studyed,The results show that disinfect for 5min suit Begonia Semperflorens,disinfect for 6min suit Begonia x hiemalis Fostsch.2.The best Inoculation Period:This research has discussed the best inoculation period of two kinds of Begonia plant,inoculation period is January,March,May,June,October,November and December,it shows that the best inoculation period of Begonia Semperflorens is November to the first ten days of December;and Begonia x hiemalis Fostsch is October.3.Primary Culture:This test study the optimum primary culture medium with three factors and three levels of the orthogonal experimental design,the optimum initiate cultivate medium for Begonia Semperflorens is 1/2MS+6-BA0.5mg/L+IBA0.8mg/L;for Begonia x hiemalis Fostseh is 1/2MS+6-BA1.0mg/L+NAA0.4mg/L.4.Successive transfer Culture:In this paper,6-BA match with NAA and IBA respectively to research the influence of hormone to proliferation of two kinds of Begonia plants.The best proliferation cultivate medium of Begonia Semperflorens is MS+6-BA0.5mg/L+NAA0.3mg/L;of Begonia x hiemalis Fostsch is MS+6-BA1.0mg/L+IBA0.5mg/L.5.Root Generating and Seedling Training Culture:Based on the result of liquid rootage culture,solid root culture was researched in detail.The result indicate that the best rootage culture medium for Begonia Semperflorens is liquid culture medium with MS+GA₃0.5mg/L+NAA0.5mg/L+sucrose30g/L;for Begonia x hiemalis Fostsch is solid culture medium with MS+IBA1.0mg/L+sucrose 20g/L. 6.Transplant and Seedling:In order to choose the best transplant method,diffenent kinds of root was watering with the second day-planting,flooding method and traditional watering method in this test,the comparison shows that the plantlet of Begonia Semperflorens obtained from liquid culture plant the second day survive most;the plantlet of Begonia x hiernalis Fostsch obtained from solid culture that withour AC suit the traditional watering method.更多还原