【作者】 宦庆松；

【导师】 范雪荣；

【作者基本信息】 江南大学 ， 纺织工程， 2009， 硕士

【摘要】 聚丙烯(Polypropylene,PP)从20世纪60年代初开始纤维级的研究与工业生产,经过90年代的高速发展,现已成为纺织工业的主要纤维之一。生产的各种制品不仅用于纺织和农业,在医疗和卫生等领域也得到大量使用,但由于是疏水性纤维需将其进行改性。国内外关于聚丙烯纤维改性的研究报导,大多集中在其表面接枝上亲水或反应性基团,而对改性后纤维上进行生物酶固定化的研究还尚不多见。本文经紫外辐照引发接枝甲基丙烯酸甲酯(MMA),在聚丙烯纤维表面引入反应性基团酯基,再通过与己二胺的酰胺化反应后用交联剂—戊二醛将改性后纤维表面的胺基与酶分子偶联,实现了生物酶在改性聚丙烯纤维上的共价固定化。主要研究内容包括光接枝反应因素对MMA接枝效果的影响,酰胺化反应及酶固定化条件对溶菌酶固定化酶活的影响。联合红外光谱(FTIR-ATR)、扫描电镜(SEM)、原子力显微镜(AFM)对聚丙烯纤维改性效果进行分析表征。最后,对聚丙烯膜的光接枝改性进行了初步的研究。实验结果表明:对聚丙烯纤维光接枝改性的最佳工艺条件为,BP用量为0.3mol/L;预照时间8min;MMA浓度为40%(v/v);紫外辐照时间:60min。改性后纤维进行酰胺化反应的较优条件为,己二胺用量30%;反应时间:2h。当采用共价交联法固定化溶菌酶时,戊二醛用量为:2%;溶菌酶:4mg/ml,固定化时间为10h。MMA的接枝效果会影响到表面胺基含量,进而对固定化溶菌酶的酶活产生影响,当接枝率为1.69%时,固定化的溶菌酶具有较高的酶活,为18U/cm2。而改性纤维在固定化溶菌酶后具有良好的抗菌性能,抑菌率为68.60%。通过对比改性前后聚丙烯纤维的FTIR-ATR、SEM、AFM与考马斯蓝染色,证实MMA确实接枝在了聚丙烯纤维表面、改性聚丙烯纤维实现了溶菌酶的固定化。光接枝改性后的聚丙烯纤维和聚丙烯膜都可以进行漆酶的固定化,且固定化后的漆酶具有一定的脱色效果。而接枝率也会对固定化漆酶的脱色效率和操作稳定性产生影响。更多还原

【Abstract】 Polypropylene from the 20th century, the beginning of the 60’s fiber-class research and industrial production, after 90 years the rapid development has become one of the main fiber of the textile industry. However, due to the non-polar polypropylene fiber material, the lack of polar molecular chain functional group, showed a certain degree of chemical inertness, and thus also to some extent limited the scope of its application. Modification of polypropylene fiber at home and abroad on research reports, most of them concentrated in the graft on the surface hydrophilic or reactive group, while the fibers on modification of immobilized enzyme research is still rare.In this paper, in order to realize enzymatic functionalization of PP fibers, PP fibers were successively modified by UV photo-induced grafting of methyl methacrylate (MMA), amidation reaction and lysozyme immobilization. The study includes light and other factors on the MMA-gg-response effect, amidation reaction and enzyme immobilization conditions on the activity of immobilized lysozyme activity. The structures and morphologies of the modified fabrics were compared using fourier transform infrared spectroscopy (FTIR-ATR)、scanning electron microscope (SEM)and atomic force microscope (AFM). Finally, we also had a preliminary study of the optical film of polypropylene grafted conducted.The result demonstrated that the optimal conditions for grafting yield on PP fiber surface were as follows: BP dosage: 0.3mol/L, according to the time pre-8min; MMA concentration of40%(v/v); UV irradiation time 60min. Modified fibers the better amidation reaction conditions was the amount of hexamethylene concentration: 30% (v/v), reaction time: 2h. When cross-linked covalently immobilized lysozyme , the amount of glutaraldehyde: 2%; lysozyme: 4mg/ml, fixed time: 10h. The effect of the grafted MMA will affect the surface amine content, and thus the activity of immobilized lysozyme have an impact, when the grafting yield was 1.69% of the lysozyme immobilized with high activity for 18U/cm2 . Modified fibers after lysozyme immobilized with good antimicrobial properties, inhibitory rate of 68.60%. By comparing before and after modified polypropylene fiber FTIR-ATR, SEM, AFM and Coomassie blue staining confirmed that MMA was grafted in polypropylene fiber surface, and modified polypropylene fiber had to achieve the immobilization of lysozyme . Photografting modified polypropylene fiber and polypropylene membrane could be immobilized laccase and immobilized laccase has some effect of decolorization and grafting yield of the immobilized laccase decolorization efficiency and impact on operational stability.更多还原