【作者】 彭娜；

【导师】 常小荣；

【作者基本信息】 湖南中医药大学 ， 针灸推拿学， 2006， 硕士

【摘要】 目的：观察艾灸足三里、梁门穴预处理对应激性溃疡(stress-induced ulcer,SU)大鼠胃粘膜损伤的影响、并与非穴组效应进行比较,证实艾灸是否通过诱导热休克蛋白70(Heat Shock Protein 70)高表达而对胃粘膜产生保护作用、是否有穴位特异性,从分子水平上进一步阐明针灸保护胃粘膜的内在机制及足阳明经与胃的密切联系,为经脉脏腑相关理论、针灸临床选穴提供实验依据。方法：将60只健康SD大鼠(雌雄各半)完全随机分为4组：空白组、模型组、足三里、梁门穴组和非穴组,每组15只。用束缚冷应激法制作应激性溃疡模型。采用Guth法计数胃粘膜损伤指数(Ulcer Index UI),生物信号分析仪检测胃粘膜血流量(Gastric Mucosa Blood Flux GMBF),免疫组织化学和逆转录聚合酶链式反应法检测胃粘膜HSP70的蛋白及基因表达(HSP70mRNA),硫代巴比妥法检测胃粘膜丙二醛(Malondialdehydein MDA)含量,放射免疫法测定胃粘膜内皮素(Endothelin ET)、前列腺素E2 (Prostaglandin E2PGE2)的含量。结果：1、与空白组比较,模型组大鼠胃粘膜UI升高、胃粘膜HSP70表达增强、GMBF和PGE2含量下降、胃粘膜MDA和ET含量升高,并有显著性差异(P<0.01或0.05)。2、与模型组比较,足三里、梁门穴组大鼠胃粘膜UI降低、胃粘膜HSP70表达增强、GMBF和PGE2含量增加、胃粘膜MDA和ET含量下降,并有显著性差异(P<0.01或0.05)。3、除胃粘膜ET含量外,足三里、梁门穴组大鼠的其余各项指标与非穴组比较均有显著性差异(P<0.01或0.05)。结论：艾灸足三里、梁门穴预处理对胃粘膜有一定的预保护作用,其机制主要体现在通过诱导胃粘膜HSP70和HSP70mRNA高表达而增加GMBF、促进胃粘膜保护性物质PGE2的释放、抑制胃粘膜损伤因子ET和MDA的释放等方面，并有穴位特异性。更多还原

【Abstract】 Objective:to explore the close relationship between the moxibustion’s effect of protecting gastric mucosa and the high expression of Heat Shock Protein 70 (HSP70) and the acupoints’relative specificity through observing the influence of the pretreatment of moxibustion on Zusanli (ST36) and Liangmen (ST21) on the stress-induced ulcer (SU) rats’gastric mucosa injury and comparing it with moxibustion on the non-acupoint.To clarify the internal mechanism of protecting the gastric mucosa of the acupuncture and moxibustion and its close relationship with Foot-Yangming meridian from the level of molecule.At the same time,to provide experimental evidence for the meridian-viscerus-correlati on theory and the clinical acupoint prescription.Methods:Sixty SD rats, both gender (by half), were evenly randomized into four groups:blank group, model group, Zusanli and Liangmen-moxibustion group and non-acupoints group.Preparing SU model using water restraint stress method, evaluating ulcer index (UI) by Guth method,determining the expression of HSP70 and HSP70mRNA of gastric mucosa with immunohistochemical and reverse transcriptase polymerase chain reaction(RT-PCR) technique,detecting the content of endothelin (ET) and prostaglandin E2 (PGE2) of gastric mucosa by radioimmunoassay and malondialdehydein (MDA) by thiobarbiturate technique.Results:1.Compared with blank group, rats’gastric mucosa UI in model group increased, the HSP70 expression and the content of MDA and ET also increased, the GMBF and the content of PGE2 decreased, with remarkable difference (P<0.01 or P<0.05).2. Compared with model group, rats’gastric mucosa UI in Zusanli-Liangmen group decreased, the HSP70 expression became more evident, the content of MDA and ET decreased, the GMBF and the content of PGE2 increased, with remarkable difference (P<0.01 or P<0.05).3. Except the content of ET, other detecting items of Zusanli-Liangmen group were remarkable different with those of non-acupoints group (P<0.01 or P<0.05).Conclusion:Moxibustion pretreatment on Zusanli and Liangmen could protect gastric mucosa. Its mechanism includes inducing gastric mucosa HSP70 and HSP70mRNA high expression, increasing GMBF, promoting PGE2’release and restraining MDA and ET producing, with the relative specificity of acupoints.更多还原