【作者】 曾姣飞；

【导师】 欧阳建军；

【作者基本信息】 湖南中医药大学 ， 方剂学， 2006， 硕士

【摘要】 目的：观察①月华胶囊对结核杆菌感染小鼠体温、体重、进食量等主要临床症状的影响；②月华胶囊对结核杆菌感染小鼠主要脏器致病指数的影响；③月华胶囊对结核杆菌感染小鼠病理形态改变的影响。方法：经尾静脉注射H37Bγ建立NIH小鼠感染结核杆菌模型。随机设立①正常组、②模型组、③月华胶囊低剂量组、④月华胶囊中剂量组、⑤月华胶囊高剂量组、⑥西药化疗(利福平+异烟肼+吡嗪酰胺)组,⑦月华胶囊联合西药化疗组。通过灌胃给药30天。观测：①主要症状改变：体温、体重、进食量；②致病指数：肺脏病变指数、脏器重量指数；③病理改变：肺、肝、脾、心、肾病理变化。结果：模型组与正常组比较各项指标差异均有显著性差异。各组之间比较：①用药各组临床症状明显优于模型组(P<0.01,P<0.05),其中联合组效果最佳,与月华胶囊各组及西药组比较差异均有显著性意义(P<0.01,P<0.05),其与正常组比较差异无显著性；月华胶囊中剂量组优于高、低两个剂量组(P<0.01,P<0.05)。②用药各组肺脏病变指数均较模型组低(P＜0.01,P<0.05),且联合组优于单独用药各组(P<0.05),单独用药各组间比较差异无显著性意义。③各用药组胸腺与肺指数明显优于模型组(P<0.01,P<0.05),联合用药组较单独用药组效果更好(P<0.05)；用药各组肝、脾指数较模型组低,用药各组之间比较无显著差异；各组肾指数比较差异无统计学意义。④用药各组与模型组比较,病理损害均有不同程度的改善(P<0.01,P<0.05)；联合用药组效果最佳,中剂量组次之,与各组之间比较有显著性差异(P<0.01,P<0.05)。结论：①月华胶囊能改善结核杆菌感染小鼠主要临床症状及致病指数,能显著降低结核杆菌对小鼠脏器的病理损害。②月华胶囊中剂量的效果最好。③月华胶囊与西药联合运用,效果优于单独用药组,说明两者间具有一定的协同作用。更多还原

【Abstract】 Objective:Observe①the effect of yuehua capsules on important symptoms such as body temperature, weight and repast of rats with tuberculosis.②the effect of yuehua capsules on disease indexes of important organs of rats with tuberculosis.③the effect of yuehua capsules on pathomorphological change of rats with tuberculosis.Methods:The NIH rats models infected tuberculosis were established by injecting H37RV into the tail vein of rats, which were grouped into the model group, western chemotherapy medicine (INH+RFP+PIA) group, Yuehua capsules high-dose group, Yuehua capsule mid-dose group, Yuehua capsule lower-dose group, Yuehua capsules & western chemotherapy medicine group, and the normal group was made before the models were built. The rats were fed by way of gavage with corresponding medicine or distilled water for 30 days continuously. The following targets were observed:①the change of important symptoms such as body temperature, body weight and food-intake.②the disease indexes such as lesion index of lung and weight index of visceral organs.③the pathomorphological change of important visceral organs such as lung, liver, spleen, kidney and heart.Results:Compared with the normal group, all targets of the model group had evident difference (P<0.01,P<0.05). Compared with every group, groups that used medicine were better than the model group(P<0.01,P<0.05), especially yuehua capsules & western chemotherapy medicine group was best among them, and compared with the normal group, there was no evident difference, but in three yuehua capsules groups, the mid-dose group was best among them(P<0.05).②Compared with the model group, the lung disease indexes of every group was lower than that of it(P<0.01,P<0.05), and the Yuehua capsules & western medicine group was better than that of groups used single medicine, in which there was no difference obviously.③Compared with the model group, the disease indexes of thymus and lung of else every group were superior to that of the model group(P<0.05),and combined group was better than that of the western medicine group or yuehua capsules groups(P<0. 05).and there was no significant difference between combined group and the model group. Compared with model group, the indexes of liver and spleen of all groups were lower than that of the model group, but there was no evident difference among groups used medicine, and there was no evident difference about kidney indexes.④Compared with the model group, there was any improvement in every groups about pathological change. The effect of Yuehua capsules & western chemotherapy medicine group was the best among groups used medicine, and the next was the mid-dose group.Conclusion:①Yuehua capsules could improve the important symptoms and disease indexes of rats with tuberculosis, and could reduce pathological damage of rats with tuberculosis.②The therapeutic effect of yuehua capsules mid-dose was the best in the three yuehua capsules groups.③The therapeutic effect of yuehua capsules & western medicine chemotherapy was better than that of groups that used only western medicine or yuehua capsules, and it was proved that yuehua capsules had a synergetic effect with western medicine.更多还原