【作者】 易亚乔；

【导师】 张炳填；

【作者基本信息】 湖南中医药大学 ， 中医临床基础， 2006， 硕士

【摘要】 目的:研究栝蒌薤白半夏汤对慢性阻塞性肺疾病(chronic obstructivepulmonary disease,COPD)气道炎症的保护作用及其作用机理。方法:采用烟熏加气管内注入脂多糖法复制COPD模型,从整体状况及肺组织病理形态学等方面观察栝蒌薤白半夏汤对COPD大鼠的保护作用;对支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)进行细胞学计数和分类检查;采用酶联免疫吸附法检测各组血清和/或BALF中细胞因子(白介素-8(interleukin 8,IL-8)、肿瘤坏死因子-α(Tumornecrosis factor alpha,TNF-α))含量。结果:1.栝蒌薤白半夏汤可使COPD大鼠整体状况、肺组织病理损害得到改善,作用强度优于或类似于泼尼松,以高剂量组为佳。2.栝蒌薤白半夏汤对COPD大鼠中小气道管壁的增厚有不同抑制作用;栝蒌薤白半夏汤可使COPD大鼠平均肺泡面积明显减少(p＜0.01);栝蒌薤白半夏汤可使大鼠BALF细胞总数、单核细胞及中性粒细胞计数比COPD组明显降低(p＜0.01),作用强度优于或类似于泼尼松,以高剂量组为佳。3.栝蒌薤白半夏汤可不同程度地降低COPD大鼠血清及BALF中IL-8、TNF-α水平(p＜0.01或p＜0.05),且高剂量组对BALF中IL-8、TNF-α作用强度优于泼尼松组。结论:IL-8、TNF-α是COPD发病的主要细胞因子之一,在COPD气道炎症中起重要作用。栝蒌薤白半夏汤对COPD气道炎症有一定治疗作用,其机理与抑制COPD气道炎症中性粒细胞聚集和活化以及抑制IL-8、TNF-α释放有关。更多还原

【Abstract】 Objective To observe the protective effects of Gualou Xiebai Banxia Decoction(GXBD) on airway inflammation of chronic obstructive pulmonary disease(COPD),and investigate its mechanism initially.Methods The rat models of COPD were established by intratracheal instillation of lipopolysaccharide(LPS) twice and exposure to cigarette smoke daily.The effects of GXBD on COPD rats were approached by observing general status(such as body weight,movement and respiration, et al) and observing the changes in pathomorhology under light micrioscope.Total and differential cell counts in bronchoalveolar lavage fluids(BALF) were examined.The levels of interleukin-8 (IL-8),tumor necrosis factor-α(TNF-α) in(BALF) or serum were measured by enzyme-linked immunosorbant assays technique(ELISA).Results After GXBD treatment,the weight of COPD rats increased significantly,movement increased and breath improved.HE staining demonstrated that the characteristics of these groups were lighter than modol group.The thickness of the middle and small airway wall of COPD rats was inhibited by GXBD.Compared with model group,the average area of alveoli in high and low dosage of GXBD groups decreased significantly(p＜0.01).The numbers of total white cell counts, neutrophils and alveolar monocyte-macrophage in BALF after GXBD intervention were significantly lower than COPD rats(P＜0.01).The levels of IL-8,TNF-αin BALE or serum were decreased in the high and low dosage of GXBD groups than COPD group(P＜0.01 or p＜0.05).Conclusion IL-8 and TNF-αmight play important roles in the airway inflammation of COPD.Gualou Xiebai Banxia Decoction might partly prevent the development of airway inflammation in COPD by inhibiting the neutrophils accumulation in the lung and reducing the release of IL-8 and TNF-α.更多还原