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捏脊疗法对脾气虚证家兔血清D-木糖及胃动素影响的研究

The Effect of Chiropractic on Content of Motilin and D-xylose in Rabbits with Spleen Qi Deficiency Syndrome

【作者】 赵锋

【导师】 袁宜勤; 李铁浪;

【作者基本信息】 湖南中医药大学 , 针灸推拿学, 2009, 硕士

【摘要】 目的:通过观察捏脊疗法对脾气虚证家兔血清D-木糖、胃动素等影响的研究,探讨捏脊对脾气虚证家兔的治疗作用,为临床捏脊疗法的应用提供实验依据。方法:将32只家兔随机分为4组:空白组、模型组、捏脊治疗组、四君子汤对照组,每组8只。各造模组(模型组、捏脊治疗组、四君子汤对照组)采用腹部注射利血平造脾气虚证模型,空白组腹部注射同等的生理盐水。造模成功后治疗组作相应治疗。分别测定各组家兔造模、治疗前后进食量、体重及血清胃动素含量、D-木糖实验中血清D-木糖含量。结果:造模后,模型组家兔行为表现活动呆滞,毛色杂暗,饮食不佳,大便稀溏,而中药组和捏脊组治疗后以上各种症状逐渐改善。治疗后,捏脊组、中药组家兔进食量、体重均有增加,与空白组比较无显著性差异(P>0.05),模型组与空白组比较有显著性差异(P<0.05),捏脊组与中药组比较无显著性差异P>0.05);捏脊组、中药组家兔血清D-木糖含量均有上升,与空白组比较无显著性差异(P>0.05),模型组与空白组比较有非常显著性差异(P<0.01),捏脊组与中药组比较无显著性差异(P>0.05),捏脊组、中药组家兔血清胃动素均有下降,与空白组比较无显著性差异(P>0.05),模型组与空白组比较有非常显著性差异(P<0.01),捏脊组与中药组比较无显著性差异(P>0.05)。结论:捏脊疗法能明显改善家兔的脾气虚症状;可能与改善家兔的胃肠运动和小肠的吸收功能有关。捏脊疗法操作简便,疗效可靠,治疗脾气虚证与四君子汤相比诸多指标无差异,值得在临床上推广。

【Abstract】 Objective:To observe the effect of chiropractic therapy on content of D-xylose and motilin in serum of rabbits with Spleen Qi Deficiency Syndrome and explore the theraputic effects of chiropractic therapy.Methods:32 rabbits were randomly divided into 4 groups:blank group, model group, chiropractic group, Sijunzi decoction group,8 in each group. Spleen-deficiency model was established by reserpine injection while rabbits in blank group were inject with normal saline. Dietary amount, weight, content of D-xylose and motilin in serum were detect after treatment.Results:The symptoms of model group were sluggish activity in rabbit behavior, miscellaneous dark color, poor diet, thin stool soft, while the Chinese medicine group and chiropractic group were with better presentations after treatment. After treatment, food intake of both chiropractic group and traditional Chinese medicine group increase, while compared with blank control group was with no significant difference (P > 0.05); the model group compared with the blank group was with a very significant difference (P<0.05); chiropractic group and Chinese medicine group was with no significant difference (P> 0.05). serum D-xylose of chiropractic group and Chinese medicine group increased, while compared with blank control group was with no significant difference (P > 0.05); the model group compared with the blank was with a very significant difference (P<0.01); chiropractic group and Chinese medicine group was with no significant difference (P> 0.05); serum MOT of both chiropractic group and the Chinese medicine group decreased, while compared with blank control group was with no significant difference (P> 0.05); the model group compared with the blank was with a very significant difference (P<0.01); chiropractic group and Chinese medicine group was with no significant difference (P> 0.05).Conclusion:Chiropractic therapy could significantly improve symptoms caused by Spleen Qi Deficiency rabbits, which might be related to the improvement of gastrointestinal motility and function of small intestinal absorption. The method of chiropractic therapy is simple and has no side effects. The effects of chiropractic therapy on Spleen-Qi Deficiency Syndrome equivalent with the effects of sijunzi decoction. The chiropractic therapy deserves the clinical expansion.

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