【作者】 王仟陆；

【导师】 苗雄鹰；

【作者基本信息】 中南大学 ， 普通外科学， 2009， 硕士

【摘要】 目的研究乳腺癌转移抑制因子1（Breast Cancer MetastasisSuppressor 1,BRMS1）和碳酸酐酶同工酶Ⅰ（Carbonic AnhydraseⅠ,CAI）在原发性肝癌（Primary Hepatic Carcinoma,PHC）组织中的表达情况,探讨它们之间的相互关系以及它们与原发性肝癌临床病理特征之间尤其是与侵袭转移之间的关系。方法收集中南大学湘雅二医院肝胆胰外科经手术切除及病理证实的原发性肝癌标本47例（术前均未进行化疗和放疗）及20例癌旁组织（距癌肿≥2cm）为对照,经10%甲醛固定后常规石蜡包埋连续切片,切片厚4μm,应用Envision^TM免疫组化染色法分别检测癌组织及癌旁组织的BRMS1蛋白和CAI蛋白的表达情况,并于高倍镜下评分。结果BRMS1蛋白和CAI蛋白在原发性肝癌的癌组织和癌旁组织均有表达,且原发性肝癌组织中BRMS1蛋白和CAI蛋白的表达阳性率和表达评分与癌旁组织相比均具有显著的统计学差异（P＜0.01,P＜0.05）。BRMS1蛋白在伴有肝内转移和癌栓形成的原发性肝癌组织中的表达阳性率及评分明显低于无肝内转移和癌栓形成的癌组织（P＜0.01,P＜0.05）,且在结节型和弥漫型肝癌中的表达阳性率明显低于巨块型肝癌（P＜0.05）,差异具有统计意义;但是,BRMS1蛋白的表达与其他各临床主要病理特征之间无明显关联（P＞0.05）。CAI蛋白在原发性肝癌组织的表达随着分化程度的降低其表达阳性率及评分逐渐升高,具有统计学意义的差异（P＜0.05）;但是CAI蛋白与其他各临床主要病理特征之间无明显关联（P＞0.05）。原发性肝癌组织中BRMS1蛋白和CAI蛋白的表达评分无明显关联（r=-0.37,P＞0.05）。结论1、BRMS1蛋白在PHC组织中的失表达在PHC的侵袭转移过程中发挥了重要的作用,但其可能对PHC的发生及生长无明显影响。2、CAI蛋白在PHC组织中的高表达在PHC的分化中发挥了重要作用,可能对肿瘤的生长及侵袭转移无明显影响。3、BRMS1蛋白和CAI蛋白在PHC组织中的表达不存在相关性,提示作为核内基因转录调控蛋白的BRMS1可能未参与CAI转录的调节。更多还原

【Abstract】 Objective To investigate the expression of Breast Cancer Metastasis Suppressor 1（BRMS1） protein and Carbonic Anhydrase I（CA I） protein in Primary Hepatic Carcinoma（PHC）,and to explore the relationships of themselves and the correlation between them and the clinicopathological characteristics of PHC.Methods The specimens from 47 patients with Primary Hepatic Carcinoma（PHC） and 20 corresponding paraneoplastic hepatic tissues were fixed in 10%formalin and routinely embedded in paraffin.The specimens were continuously sliced into 4μm-thick sections.Envision^TM immunohistochemistry was performed to detect the expressions of BRMS1 protein and CA I protein with monoclonal antibodies and scored them under high-power microscopy.Results In the 47 Primary Hepatic Carcinoma（PHC） cases,there are significantly statistical distinctions in the expressive positive rate and the expressive scores of BRMS1 and CA I protein between cancer tissues and paraneoplastic tissues（P＜0.01,P＜0.05）The cancer tissues with intrahepatic metastasis and tumor thrombosis have significantly lower expressive positive rate and expressive scores of BRMS1 protein than the others（P＜0.01,P＜0.05）,and also there is a sinificantly lower positive rate in nodular and diffuse PHC than massive ones（P＜0.05）.But we haven’t found the relatioships between BRMS1 and other main clinicopathological characteristics of PHC（P＞0.05）.There is no correlation between the expression of CA I protein and clinicopathological characteristics of PHC,except that the expressive positive rate and the expressive scores of PHC significantly gradually increase with the descending differentiation degree（P＜0.05）.There is no evident correlation between the expressive scores of BRMS1 protein and that of CA I protein in cancer tissues of PHC（r= -0.37,P＞0.05）Conclusion The lost expression of BRMS 1 protein in PHC plays an impotant role in the invasive and metastatic process,without effect on the transformation and growth of PHC.On the contrary,the overexpression of CA I protein in PHC is closely related to the differentiation,and it may have nothing to do with the growth、invasiveness and metastasis.And there is no correlation between the expressive scores of BRMS1 protein and that of CA I protein in cancer tissues of PHC更多还原