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HIF-1α和VEGF在垂体腺瘤中的表达和意义
Expressions and Their Significance of HIF-1a and VEGF in Pituitary Adenomas
【作者】 敖红敏；
【导师】 张剑；
【作者基本信息】 南昌大学 ， 耳鼻咽喉—头颈外科学， 2009， 硕士
【摘要】 目的:检测缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)在侵袭性和非侵袭性垂体腺瘤中的表达,探讨其与肿瘤侵袭发生的关系及它们之间的相关性。方法:采用免疫组化Envision二步法检测56例垂体腺瘤,其中32例侵袭性垂体腺瘤及24例非侵袭性垂体腺瘤标本中HIF-1α和VEGF的表达。结果: HIF-1α在侵袭性垂体腺瘤中的强阳性及阳性表达率分别为53.1%和93.8%,明显高于非侵袭性垂体腺瘤中的16.7%和50.0%(P<0.01);VEGF在侵袭性垂体腺瘤中的强阳性及阳性表达率分别为56.3%和96.9%,明显高于非侵袭性垂体腺瘤中的12.5%和45.8%(P<0.01)。侵袭性腺瘤组中HIF-1α与VEGF的表达成正相关(P<0.01),非侵袭性腺瘤组中HIF-1α与VEGF的表达无明显相关性。结论: HIF-1α、VEGF高表达与垂体腺瘤的侵袭性密切相关,可以作为判别垂体腺瘤侵袭性的有效参考指标。HIF-1α、VEGF在肿瘤侵袭及新生血管形成过程中可能存在协同作用。
【Abstract】 Objective: To investigate the expressions of HIF-1αand VEGF in patients with invasive pituitary adenomas,and to evaluate the relationship between the expression of HIF-1αand VEGF,and the invasion of pituitary adenomas.Methods: Immunohistochemical Envision method was used to determine the expressions of HIF-1αand VEGF in 56 cases of pituitary adenomas,32 cases were invasive and 24 were non-invasive patients with pituitary adenomas.Result: The intense expression and expression rates of HIF-1αof pituitary adenomas patients with invasion were significantly higher than those without invasion(53.1% vs 16.7% and 93.8% vs 50.0%,p<0.01). The intense expression and expression rates of VEGF of pituitary adenomas patients with invasion were significantly higher than those without invasion(56.3% vs 12.5% and 96.9% vs 45.8%,p<0.01).There was a mutually positive correlation among HIF-1αand VEGF expressions in invasive pituitary adenomas(P<0.01),and no correlation among HIF-1αand VEGF expressions in non-invasive pituitary adenomas.Conclusion: The high expression of HIF-1αand VEGF might relate to the aggressiveness and invasion in pituitary adenomass and may be useful markers for predicting invasiveness, and they might have close relationship in the invasiveness and angiogenesis of pituitary adenomas.