【作者】 徐俊；

【导师】 秦治明；

【作者基本信息】 重庆医科大学 ， 外科学， 2009， 硕士

【摘要】 目的探索分化抑制因子（Inhibitors of differentiation,Id）Id₁和Id₃在非小细胞肺癌中的表达情况,分析其与肺癌的淋巴结转移,及与患者预后等的关系。方法收集重庆医科大学第一附属医院胸心外科2004年至2007年非小细胞肺癌病人术后的石蜡标本60例,根据其年龄、性别,肿瘤的病理学类型,有无淋巴结转移情况等,分别进行分组对照。术后病理结果均经重庆医科大学病理科诊断证实。继而应用免疫组化SP法测定标本中Id₁和Id₃的表达情况,按SP试剂盒介绍的方法进行。Id₁、Id₃阳性信号为胞浆内出现黄色至棕褐色染色,采用染色强度×染色细胞百分率综合记分方式评估,将不同组别中Id₁和Id₃的表达情况进行对照分析,并将所有非小细胞肺癌样本中Id₁和Id₃的表达情况进行相关性分析.结果在人非小细胞肺癌组织中,Id₁和Id₃均过度表达,与正常肺组织及良性病变组织（结核和炎性假瘤）比较差异具有显著性（P<0.01）;在不同年龄、性别及病理类型中表达的差异无统计学意义（P>0.05）;在肿瘤有淋巴结转移组中表达的明显高于无淋巴结转移组,统计分析差异具有显著性（P<0.05）。相关性分析示:Id₁和Id₃在非小细胞肺癌中表达呈正相关。结论Id₁、Id₃蛋白与非小细胞肺癌的发生发展及淋巴结转移有关,而与患者的年龄、性别、肿瘤的病理类型等无关。Id₁和Id₃在非小细胞肺癌中正协同表达参与了肿瘤的发生。目前认为有无淋巴结转移是决定肺癌分期、治疗方法及判断预后的重要参照指标,因此,Id₁和Id₃蛋白可能作为非小细胞肺癌诊断、决定治疗方案及判断预后的重要标志物之一,这为非小细胞肺癌的诊断和治疗提供了新的研究思路。更多还原

【Abstract】 Objective: To study the expression of Id₁ and Id₃ in human non-small cell lung cancer （NSCLC）, and to analyze its potential clinical significance.Methods: 60 paraffin-embedded specimens were collected and the specimens were got from the patients with NSCLC in the first affiliated hospital of Chongqing Medical University from 2004 to 2007. The cases were separately grouped according to the age, gender, pathological type of tumor, lymph node metastasis, etc. The pathological results were all confirmed by the department of pathology of Chongqing Medical University. Then, the expression of Id₁ and Id₃ were detected by immunohistochemical staining technique. The positive signals of Id₁ and Id₃ were the yellow or brown staining in intracytoplasm. We used the method of staining intensity×staining cells to analyze the results. We had done the contrast analysis to find out the reasons of the different expression of Id₁ and Id₃ in each group and the correlation analysis to find out the relationship of Id₁ and Id₃ in all NSCLC samples.Results: There were no Id_l or Id₃ expressions in normal lung tissue, Negative to weak expression of those could be observed in benign disease tissues. However, the expressions of Id₁ and Id₃ were obviously strong in NSCLC, which was significantly higher than that in benign disease （P<0.01）. Furthermore, the over expressions of Id₁ and Id₃ significantly correlated with lymph node metastasis （P<0.05） ,and they were not related to pathological type, the age or the gender of the patients（P>0.05）. The correlation analysis showed positive correlation of them.Conclusion: Id_l and Id₃ protein are overexpressed in NSCLC and the positive expression is correlated with lymph node metastasis. It suggested that Id_l and Id₃ are associated with tumorigenesis, invasion and metastasis in NSCLC. Id_l and Id₃ might be used as new markers of diagnosis and prognosis for NSCLC.This finding might put forward a new method for NSCLC treatment.更多还原