【作者】 贾春娟；

【导师】 王朝晖；

【作者基本信息】 大连医科大学 ， 内科学， 2009， 硕士

【摘要】 目的:探讨PTEN与COX-2在大肠癌组织中的表达及与大肠癌生物学特性的关系。方法:应用免疫组织化学染色法对48例大肠癌组织、20例大肠腺瘤组织和20例正常大肠粘膜组织进行免疫组织化学染色,COX-2以试剂公司提供的阳性表达的结肠癌切片作为阳性对照, PTEN以试剂公司提供的阳性表达的扁桃体切片作为阳性对照,以PBS置换一抗作为阴性对照。结果采用阳性细胞数和染色强度得分相乘法,分别计为“-、+、++、+++”。按此等级应用χ2检验分析COX-2和PTEN的表达情况及与大肠癌生物学特性的关系;并应用Spearman检验分析COX-2与PTEN在大肠癌中表达的关系。结果:1. COX-2表达定位于细胞胞浆中,大肠癌、大肠腺瘤、正常大肠组织中的表达率分别为79.2%、50.0%、15.0%,各组间比较差异有统计学意义(P<0.05)。2. COX-2表达与大肠癌生物学特性的关系:COX-2在大肠癌中的表达与淋巴结转移、Dukes分期有相关性,而与性别、年龄、肿瘤大小、肿瘤部位及肿瘤组织分化程度无相关性。大肠癌组织中COX-2在淋巴结转移中的表达率91.7%明显高于无淋巴结转移的66.7%,差异有统计学意义(P<0.05);DukesC、D期COX-2的表达率(92.3%)明显高于DukesA、B期的表达率(63.6%),差异有统计学意义(P<0.05)。3. PTEN表达定位于细胞核,大肠癌、大肠腺瘤、正常大肠粘膜组织中的表达率分别为33.3%、60.0%、95.0%,各组间比较差异有统计学意义(P<0.05)。4. PTEN表达与大肠癌生物学特性的关系: PTEN在大肠癌中的表达与淋巴结转移及Dukes分期相关,而与性别、年龄、肿瘤大小、肿瘤部位及肿瘤组织分化程度无相关性。伴淋巴结转移中PTEN的表达率为14.6%,无淋巴结转移中PTEN的表达率为50.0%,两组间差异有统计学意义(P<0.05)。DukesC、D期的表达率为19.2%,A、B期的表达率为50.0%,两组间比较差异有统计学意义(P<0.05)。5.PTEN和COX-2在大肠癌中表达的相关性本组38例COX-2表达阳性的标本中,PTEN阳性表达9例,阴性表达29例;10例COX-2表达阴性的标本中,PTEN阳性表达7例,阴性表达3例;二者呈负相关,r=-0.326, P<0.05。结论:1.COX-2在大肠癌组织中的表达率明显高于其在大肠腺瘤、正常大肠粘膜组织中的表达。COX-2在大肠癌中的表达与淋巴结转移和Dukes分期相关,而与性别、年龄、肿瘤大小、肿瘤部位及肿瘤组织分化程度无相关性。2.PTEN在大肠癌组织中的表达率明显低于其在大肠腺瘤、正常大肠粘膜组织中的表达。PTEN在大肠癌中的表达与淋巴结转移及Dukes分期相关,而与性别、年龄、肿瘤大小、肿瘤部位及组织分化程度无相关性。3.COX-2和PTEN在大肠癌组织中的表达呈负相关( r=-0.326,P<0.05),提示COX-2和PTEN在大肠癌发生发展过程中起重要的负向协同作用。更多还原

【Abstract】 Objective: To investigate the expressions of COX-2 and PTEN in colorectal carcinoma and their correlations with the bionomics of colorectal carcinoma.Methods: The expressions of COX-2 and PTEN protein were deter- mined by immunohistochemical staining (Streptavidin-Peroxidase/ S-P method) in the tissues of 48 colorectal carcinoma, 20 colorectal adenomas and 20 normal colorectal mucous .Based on dying strength and masculine cell population ,the results were divided as“-,+,++,+++”. The data was analyzed by software SPSS11.5. The difference of COX-2 and PTEN expression in the different pathological classifications of colorectal carcinoma was analyzed with Chi-square Test. Spearman method was used to analyze the relationship between COX-2 and PTEN expression in the colorectal carcinoma.Results: (1).The positive staining of COX-2 mainly located in cytoplasm. The rate of COX-2 expression was 79.2% in colorectal carci- noma group, 50.0% in colorectal adenoma and 15.0% in the normal colore- ctal mucous membrane group respectively. There were significant differences among the there groups (P<0.05). The expression of COX-2 had relationship with lymphatic metastasis and Dukes stages, but had no relationship with sexuality, age,size ,position and histological differentia- tion. The rate of COX-2 expression was 91.7% in colorectal carcinoma group with lymphatic metastasis and 66.7% in colorectal carcinoma group without lymphatic metastasis. A significant difference was observed between the two groups (P<0.05). The rate of COX-2 expression was higher in the group of Dukes C and D stages than that in the group of Dukes A and B stages (P<0.05).(2).The positive staining of PTEN mainly located in the nucleus of colorectal carcinoma tissues. The rate of PTEN expression was 33.3% in colorectal carcinoma group, 60.0% positive expression in the colorectal adenoma and 95.0% positive expression in normal colorectal mucous membrane group respectively. There were statistically significant differences among the there groups (P<0.05). The expression of PTEN had relationship with lymphatic metastasis and Dukes stages, but had no relationship with sexuality, age, size, position and histological differentia- tion. The rate of PTEN expression was 14.6% in colorectal carcinoma group with lymphatic metastasis and 50.0% in colorectal carcinoma group without lymphatic metastasis. A significant difference was observed between the two groups (P<0.05).The rate of PTEN expression was 50.0% in the group of Dukes A and B stages and 19.2% in the group of Dukes C and D stages. The positive expression rate of A and B stages was higher than that of C and D stages (P<0.05). (3).Of the 38 specimens with positive COX-2,9 had the positive expression of PTEN; of the 10 specimens with negative COX-2,7 had the positive expression of PTEN. There was a negative correlation between COX-2 and PTEN(r=-0.326, P<0.05).Conclusion:(1). COX-2 is highly expressed in colorectal carcinoma tissues,but it is lowly expressed in adenoma and normal tissues. The expression of COX-2 is related to lymphatic metastasis and Dukes stages. But it has no relationship with sexuality, age,size ,position and histological differentiation. (2). PTEN is lowly expressed in colorectal carcinoma and colocrectal adenoma tissues, but it is highly expressed in normal colorectal mucous membrane. The expression of PTEN has relationship with lymphatic metastasis and Dukes stages ,but it is not related to sexuality, age,size ,position and histological differentiation. (3). COX-2 and PTEN play an important role in the carcinogenesis of colorectal carcinoma. The expression of COX-2 is negative related to that of PTEN in colorectal carcinoma tissues.更多还原