【作者】 盛华；

【导师】 曹东升；

【作者基本信息】 安徽医科大学 ， 外科学， 2009， 硕士

【摘要】 目的探讨瘤体注射平内阳霉素对增殖性血管瘤血管内皮细胞生长因子(VEGF)、碱性成纤维细胞长因子(bFGF)和增殖细胞核抗原(PCNA)表达水平的影响及可能机制。方法增殖血管瘤140例随机分成7组,每组20例,A组作为对照组;B组平阳霉素(1mg/ cm2)术前1周瘤体注射;C组平阳霉素加地塞米松术前1周瘤体注射; D组平阳霉素术前2周瘤体注射;E组平阳霉素加地塞米松术前2周瘤体注射; F组平阳霉素术前3周瘤体注射;G组平阳霉素加地塞米松术前3周瘤体注射。对收集到的血管瘤标本采用组织化学方法和免疫组织化学方法(SP法),进行光镜下组织结构观察及VEGF、PCNA和bFGF的含量检测。结果A、F、G组VEGF的表达水平为78.00±3.57,75.90±2.63,70.54±3.11,B、C、D、E组为56.35±2.60,41.50±2.12,40.10±2.27,27.70±1.40差异有显著性(P<0.05)。A、B、F、G组bFGF的表达水平81.40±2.00,73.55±1.67, 71.45±3.88,69.70±3.76, C、D、E组53.65±2.29,45.35±1.82,31.90±2.55,差异有显著性(P<0.05)。A、F、G组PCNA的表达水平为79.55±3.15, 76.30±3.71,69.20±2.97, B、C、D、E组57.30±2.34,44.95±2.47,44.25±1.60,34.00±2.25,差异有显著性(P<0.05)。结论平阳霉素瘤体注射可明显抑制血管瘤组织中VEGF和bFGF的表达,从而抑制其增殖。平阳霉素与糖皮质激素(地塞米松)联合应用局部注射能起到协同作用,术前2周局部注射平阳霉素或联合激素可缩小血管瘤的体积,减少术中出血,减小术后创面。更多还原

【Abstract】 Objective To explore the influence of intratumoral injection of pinyangmycin on the expression levels of VEGF,PCNA and bFGF in proliferating hemangioma. methods One hundred and forty patients with proliferating hemangioma were randomly divided into seven groups, twenty patients every group .Group A as control group. Group B were injected with Pinyangmycin(1mg|ml) before 1 week .Group C were injected with pinyangmycin and dexamethasonum before one week. Group D were injected with Pinyangmycin before two weeks. Group E were injected with pinyangmycin and dexamethasonum before two weeks. Group F were injected with Pinyangmycin before three weeks. Group G were injected with pinyangmycin and dexamethasonum before three weeks. Observe the tissue configuration of above examples by histochemistry method and detect the expression level of the VEGF,bFGF and PCNA by immunohistochemistry method. Results The expressions of VEGF in the A,F,G groups were 78.00±3.57,75.90±2.63,70.54±3.11 and in the B,C,D,E groups were 56.35±2.60,41.50±2.12,40.10±2.27,27.70±1.40.The variance was significance(P<0.05). The expressions of bFGF in the A,B,F,G groups were 81.40±2.00,73.55±1.67, 71.45±3.88,69.70±3.76 and in the C,D,E groups were 53.65±2.29,45.35±1.82,31.90±2.55, The variance was significance(P<0.05). The expressions of PCNA in the A,F,G groups were 79.55±3.15, 76.30±3.71,69.20±2.97 and in the B,C,D,E groups were 57.30±2.34,44.95±2.47,44.25±1.60,34.00±2.25 The variance was significance(P<0.05). Conclusion Intratumoral injection of pinyangmycin in proliferating hemangioma may obviously restrain the expressions of VEGF and bFGF to inhibit it proliferation. Dexamethasonum and Pinyangmycin enhance role of each other . intratumoral injection of Pinyangmycin and Dexamethasonum before operating will shrink area of hemangioma.decrease demage of operation to body.更多还原