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四翅滨藜叶蛋白的提取及物性学研究

Study on Extracting Techniques and Physical Properties of Leaf Protein from Atyiplex Canescen

【作者】 王桓

【导师】 敬思群;

【作者基本信息】 新疆大学 , 食品科学, 2009, 硕士

【摘要】 本文对四翅滨藜(Atyiplex Canescen)叶蛋白的提取及物性学进行了试验研究,其主要研究结果如下:1、通过对酸化加热法、直接加热法、碱化加热法、酸提法、碱提法和超声提取的比较试验,确立酸化加热法作为本研究的试验方法,其最佳工艺流程为:四翅滨藜→加水、浸提、打浆(料液比1∶20,打浆时间4min)→离心(3000r/min,20min)→绿色汁液→絮凝(pH3.0,加热温度60℃,5min)→离心(4000r/min,10min)→叶蛋白膏→冷冻干燥→叶蛋白。2、对四翅滨藜叶蛋白进行营养成分分析,结果表明,四翅滨藜叶蛋白粗蛋白含量达57.69%,高于苜蓿叶蛋白、大豆饼,与鳀鱼鱼粉中粗蛋白质含量相当,粗脂肪、粗灰分等其他常规营养成分也均符合FAO/WHO标准,必需氨基酸含量完善。还含有丰富的Ca、P等矿质元素及Cu、Fe、Mn和Zn等微量元素,具有很高的饲用价值。3、对四翅滨藜蛋白的功能性质进行了研究,结果表明,四翅滨藜蛋白的功能性质均好于大豆分离蛋白和苜蓿蛋白,可替代大豆蛋白粉应用于食品之中。4、对提取叶蛋白后的草渣和茎干进行营养成分分析,结果表明草渣和茎干均有较高的粗蛋白含量(分别为16.26%和10.86%)。其他常规营养成分接近于二级的饲料用干草,可作为家畜优良的饲草资源。

【Abstract】 The extracting techniques and physical properties of leaf protein from atyiplex canescen were studied.The main resultes are described as follow:1 The acidified thermal method,the thermal method,alkalized thermal method,acid and alkali method and ultrasonic method for extracting leaf proteins from atyiplex canescen are observed comparatively.The results showed that the best method in acidified thermal method and the optimal technical processing were: atyiplex canescen→adding water,soaking,refining (the ratio of material and wanter is 1:20,refining time is 4 min)→centrifugal casting (3000r/min,10min)→green juice→flocculation(pH3.0,heating temperature 6℃,for 5min)→centrifugal casting(4000r/min,10min)→cencretion of leaf protein→freeze-drying→leaf protein of atyiplex canescen.2 Nutrition components of atyiplex canescen leaf protein was analysed,The results showed that crude protein content of atyiplex canescen leaf protein was 57.69% and higher than or at least near to the alfalfa leaf protein’s,bean cake’s and fish meal’s.The crube fat,crube ash and several kinds of nutrition components were also accord with FAO/WHO standards.Contents of essential amino acid are consummate. Atyiplex canescen leaf protein was rich in Ca, P and other mineral elements and Cu,Fe,Mn,Zn and other trace elements.So atyiplex canescen leaf protein has the high feeding value.3 Functional properties of atyiplex canescen leaf protein was studied, The results showed that functional properties of atyiplex canescen leaf protein were better than soy protein isolate and alfalfa leaf protein and could be applied to food instead of soy protein isolate.4 Nutrition components of atyiplex canescen stem and grass residue from the extracting process were analysed,The results showed that nutrition content in the grass residue was comparable to the second-level atyiplex canescen hay,and could turn into excellent foeage grass resource of critter.

  • 【网络出版投稿人】 新疆大学
  • 【网络出版年期】2010年 01期
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