【作者】 陈宝；

【导师】 金其贯； 李宁川；

【作者基本信息】 扬州大学 ， 运动人体科学， 2009， 硕士

【摘要】 目的:探讨有氧运动联合膳食控制对2型糖尿病大鼠血清visfatin水平的影响,并分析其他相关指标的变化。方法:选用6周龄雄性SD大鼠62只,大鼠随机抽取8只大鼠作为正常对照组(Control,C组),喂以标准普通饲料。其余54只SD大鼠在喂饲高糖高脂膳食的基础上,腹腔注射小剂量的链脲佐菌素(streptozotocin,STZ),建立2型糖尿病动物模型。然后将2型糖尿病大鼠随机分成4组:DM对照组(DM Control group,DM,n=9)、DM+运动锻炼组(DM+Exercise training group,DME,n=10)、DM+膳食控制组(DM+Diet control group,DMD,n=10)、DM+运动锻炼+膳食控制组(DM+Exercise training + Diet control,DMED,n=10)。DM组大鼠继续喂饲高脂高糖饲料,不进行运动锻炼;运动锻炼采用每天进行60min的无负重游泳运动,每周6次;膳食控制采用与DM组等量的标准普通饲料。13周后,检测各组大鼠FPG、TG、TC、FINS和Visfatin的含量。结果:①2型糖尿病大鼠模型建立后第13周,与C组相比,DM组大鼠的FPG显著升高(P<0.01);通过双因素方差分析,有氧运动可以降低糖尿病大鼠FPG水平(P<0.05),而膳食控制对糖尿病大鼠FPG水平没有显著性影响(P>0.05),有氧运动联合膳食控制对降低糖尿病大鼠FPG水平没有显著性交互作用(P>0.05)。②2型糖尿病大鼠模型建立后第13周,DM组大鼠与C组大鼠相比,血清胰岛素浓度显著降低(P<0.01),但HOMA-IR无显著性变化(P>0.05)。通过双因素方差分析,膳食控制可以显著性提高糖尿病大鼠FINS水平(P<0.05),但有氧运动和有氧运动联合膳食控制可使糖尿病大鼠FINS水平进一步升高,但无显著的交互作用(P>0.05);有氧运动或膳食控制对DM大鼠的HOMA-IR均无显著性影响(P>0.05),且两者无显著的交互作用(P>0.05)。③2型糖尿病大鼠模型建立后第13周,DM组大鼠与C组大鼠相比,血清内酯素浓度显著升高(P<0.01);通过双因素方差分析,13周的中强度的游泳训练对降低糖尿病大鼠血清内脂素含量有显著性的影响;与DM组相比,虽然DMD组血清内脂素含量降低很多,但通过双因素方差分析,膳食控制对降低糖尿病大鼠血清内脂素含量无显著性影响(P>0.05);而有氧运动和膳食控制可以进一步降低糖尿病大鼠的血清visfatin浓度,但无显著的交互作用(P>0.05)。④2型糖尿病大鼠模型建立后第13周,与C组相比,DM组血清总胆固醇浓度和甘油三酯浓度显著升高(P<0.01);通过双因素方差分析,有氧运动或膳食控制均能显著地降低糖尿病大鼠的TC浓度(P<0.05),而且有氧运动和膳食控制对降低糖尿病大鼠的TC浓度具有明显的交互作用(P<0.05);膳食控制能显著地降低糖尿病大鼠的TG浓度(P<0.01),有氧运动并不能使糖尿病大鼠的TG浓度显著降低,而且,有氧运动联合膳食控制虽然可使糖尿病大鼠的TG浓度进一步降低,但没有显著的交互作用(P>0.05)结论:①本研究所建立的糖尿病模型是成功的;在糖尿病的治疗中,单纯地依靠膳食控制并不能有效地降低糖尿病大鼠的FPG,而有氧运动对控制糖尿病大鼠的FPG具有非常显著的作用,在膳食控制的基础上进行有氧运动对降低糖尿病大鼠的血糖水平更加有效。与此同时,2型糖尿病大鼠血清visfatin水平显著升高。可能是2型糖尿病发生发展过程中对胰岛素分泌下降,血糖水平升高的一种代偿机制。②13周的有氧运动能够增加胰岛β细胞分泌胰岛素的能力;单纯的膳食控制能有效地增加糖尿病大鼠的FINS的含量;在膳食控制的基础上进行有氧运动对降低糖尿病大鼠的血糖水平更加有效,对2型糖尿病的治疗具有非常重要的作用,但无显著的交互作用。③有氧运动可使糖尿病大鼠血清visfatin的水平显著降低;13周的膳食控制对降低2型糖尿病大鼠血清visfatin水平影响不大;有氧运动联合膳食控制虽然能使2型糖尿病大鼠血清visfatin含量进一步降低,但没有显著的交互作用;且血糖水平与血液中visfatin水平之间有着密切的关系,可能是调节visfatin合成和分泌的主要因素。④有氧运动、膳食控制以及有氧运动联合膳食控制对改善2型糖尿病大鼠的脂代谢具有积极的作用。与此同时,血清visfatin水平对血脂代谢的调节也具有一定的作用,但是它们之间的确切关系还有待于进一步研究。更多还原

【Abstract】 To explore the effect of aerobic exercise joint dietary control on the visfatin plasma levels in type 2 diabetic rats and analyze the changes in other relevant indicators. Method:Chose 62 single of 6-week-old SD rats which were male, Eight rats were randomly selected as normal control group of rats (Control, C group), and fed with normal standard diet. The remaining 54 SD rats which were feed high-fat and high-sugar diet were injected of low-dose streptozotocin though abdominal cavity to establish animal models of type 2 diabetes. and then the rats of type 2 diabetes were randomly divided into 4 groups : DM control group (n=9), DM+ aerobic exercise training group (n=10), DM+ Diet control group (n=10), DM+ aerobic exercise training + Diet control group (n=10). The DM group’s rats which were continued to feed high-fat and high-sugar diet were not exercise; Exercise were used 60 minutes’unloaded swimming for 13 weeks, 6 times per week; The dietary control use the general contour of the standard diet of the DM group. 13 weeks later, FPG, TG, TC, FINS, Visfatin of every group’s rats were examined. Results:①After 13 weeks of the establishment of type 2 diabetes model, compared with C group, the FPG contents increased significantly in DM group(P<0.01); Through the double factor variance analysis, aerobic exercise can lower FPG levels in diabetic rats (P<0.05), And the effect of dietary control on FPG levels in diabetic rats did not significantly(P>0.05), exercise joint dietary control did not display an obvious interaction on lowering FPG levels in diabetic tats(P>0.05). ②After 13 weeks of the establishment of type 2 diabetes model, compared with C group, the serum insulin concentration decreased significantly in DM group(P<0.01), but the HOMA-IR was no significant changes. Through the double factor variance analysis, diet control can increase the FINS level in diabetic rats significantly(P<0.05), aerobic exercise and aerobic exercise joint dietary control in diabetic rats could further increase the level of FINS, but no significant interaction(P>0.05); there was neither significantly effect of aerobic exercise nor dietary control to improve the DM group of HOMA-IR(P>0.05), and there was no significant interaction(P>0.05).③After 13 weeks of the establishment of type 2 diabetes model, compared with C group, the visfatin plasma concentrations significantly increased(P<0.01); Through the double factor variance analysis, there was significant impact of 13 weeks of strength training for swimming to lower plasma visfatin concentrations in diabetic rats; compared with the DM group, Although the visfatin plasma concentration lowered too much in DMD group, through the double factor variance analysis, there was no significant impact of dietary control to lower the visfatin plasma concentration(P>0.05); aerobic exercise and diet control can further reduce the visfatin plasma concentration in diabetic rats,and there was no significant interaction(P>0.05).④After 13 weeks of the establishment of type 2 diabetes modle, compared with C group, the serum total cholesterol concentration and the serum triglyceride concentration increased significantly(P<0.01); Through the double factor variance analysis, both of aerobic exercise and dietary control could significantly lower the total cholesterol concentration in type 2 diabetic rats(P<0.05), and there was significant interaction of both aerobic exercise and dietary control to lower the total cholesterol concentration in type 2 diabetic rats(P<0.05). Dietary control could lower the triglyceride concentration in type 2 diabetic rats(P<0.01)significantly, but aerobic exercise could not , although aerobic exercise joint dietary control could further lower the triglyceride concentration in type 2 diabetic rats, there was no significant interaction (P>0.05).Conclusion:①Establishing of the Model of type 2 diabetes in this research was successful. Dietary control only could not effectively lower the FPG concentration; And aerobic exercise training has a very significant role to control the FPG of diabetic rats, on the base of the diet control, aerobic exercise training was more effective to reduce the fasting plasma glucose levels which in diabetic rats. At the same time, the visfatin plasma levels which in type 2 diabetic rats was significantly increased.②13 weeks of aerobic exercise training could improve the capacity of which Pancreaticβ-cell secreted insulin in type 2 diabetes rats; Although aerobic exercise joint dietary control could increase the insulin concentration in type 2 diabetes rats, and it had a very important role in the treatment of type 2 diabetes, there was no significant interaction.③Aerobic exercise could significantly lower the level of visfatin plasma in type 2 diabetic rats ; There was little effect on lowering the visfatin plasma concentration of 13 weeks of dietary control in type 2 diabetic rats; Aerobic exercise joint dietary control could further lower the visfatin plasma content in type 2 diabetes rats, but there was no significant interaction; And there was a close relationship between blood glucose levels and visfatin levels. It might be a main factor on regulating the visfatin’s synthesis and secretion.④It was an active role in improving lipid metabolism for aerobic exercise training、dietary control and aerobic exercise joint dietary control. At the same time, it was also a role in regulating the lipid metabolism for visfatin plasma levels, However, the precise relationship between them had to be further studied.更多还原