【作者】 胡述博；

【导师】 徐瑞荣；

【作者基本信息】 山东中医药大学 ， 中西医结合临床， 2009， 硕士

【摘要】 目的:本研究旨在通过研究中药复方干预白血病细胞FLT3和NF-κB的蛋白表达情况,以探讨中医药治疗白血病的作用机制。方法:运用中药对45例AML患者白血病干细胞进行干预,通过免疫印迹法(Western Bloting,WB)观察干预前后白血病干细胞内NF-κB和FLT3的表达情况;同时观察患者的性别、年龄、治疗前外周血白细胞数及骨髓原始细胞比例,以探讨其相关性。结果:AML患者(M1、M2、M4、M5)FLT3阳性率为62.2%;FLT3的阳性率与患者的FAB分型、性别、年龄无差异(P>0.05),与患者的外周血白细胞总数、骨髓原始细胞比例存在差异(P<0.01);中药可下调白血病干细胞FLT3和NF-κB基因的蛋白表达水平(P<0.01);其中中药原方组与其拆方扶正组、祛邪组及生理盐水组(空白对照组)相比,能够显著降低白血病干细胞FLT3和NF-κB基因的蛋白表达水平(P<0.01),而扶正组与祛邪组对白血病干细胞FLT3和NF-κB基因表达水平的影响无显著性差异(P>0.05)。结论:AML患者中存在FLT3的阳性表达;其阳性表达率与患者的年龄、性别、FAB分型无关,与患者初发或复发时外周血白细胞计数及骨髓原始细胞数成正相关;中药复方可以下调FLT3及NF-κB在AML患者中的表达,其中原方组较祛邪组、扶正组下调作用明显;揭示了扶正组与祛邪组之间存在协同作用。更多还原

【Abstract】 Objective:To research changes of proteinic expression in NF-κB (Nuclear Factor- Kappa B)and FLT3(Fms-like tyrosine kinase 3),and the mechanism of herbs to acute myelocytic leukemia cell(AML).Methods: BM(bone marrow)of forty-five AML patients were extracted to get monocytes .After that, by magneton selecting we can get leukemia stem cells.The third step is cultivating the LSC by four different drugs:Yuanfang group,Fuzheng group,Quxie group and 0.9% NaCl.Then we can obtain protein of LSC. The last is getting the protein expressions by western blotting(WB)and analyzing the changes to summarize the mechanisms of NF-κB and FLT3 by herbs.And observe sex,ages,the WBC and aboriginal cells of BM of AML patients to research the relativity. Results: The expression of FLT3 is not existing differents in different ages and sex of AML patents(P>0.05).But you can get the result that there exists differents in different WBC and aboriginal cells of BM of AML patients(P<0.01). The proportion of FLT3 expression is 62.2 %. The proteinic expressions of NF-κB and FLT3 after different drugs treatment are have dissimilarity between random two groups(P<0.01)except Fuzheng group and Quxie groups(P>0.05). Conclusion: Positive expression of FLT3 in AML patients which are not existing relativity in ages and sex ,but has relativities with WBC and aboriginal cells of BM. Formula Herbs can reduce the exoression of FLT3 and NF-κB. And Yuanfang group can obviously resuce the expression comparing with Fuzheng group and Quxie group. Fuzheng group and Quxie group treatment effect are better when using together.更多还原