【作者】 林克旭；

【导师】 刘楠；

【作者基本信息】 福建医科大学 ， 神经病学， 2009， 硕士

【摘要】 目的探讨IL-10基因启动子区域- 1082、- 819和- 592位点单核苷酸多态性与脑梗死的关系。方法用扩增受阻突变系统-聚合酶链反应(ARMS-PCR)技术,对181例脑梗死患者(脑梗死组)和115名健康者(对照组)的IL-10基因启动子区域- 1082A/G、- 819C/T和- 592A/C位点核甘酸多态性进行分析。结果脑梗死组IL-10-1082A/G基因型的分布频率与对照组比较,差异有统计学意义(χ~2=6.643 P=0.010);A等位基因频率为92.3%,对照组为86.1%,两组比较差异有统计学意义(P=0.015);IL-10-819C/T和IL-10-592C/A的基因型分布频率及等位基因频率与对照组比较差异无统计学意义(P>0.05)。结论IL-10-1082A/G基因多态性与脑梗死的发病有关,其中A等位基因频率增高与脑梗死发病有关。更多还原

【Abstract】 Object To investigate whether three diallelic polymorphisms at the position - 1082,- 819 and - 592 in the promoter region of the IL-10 gene were associated with cerebral infarction(CI).Methods The IL-10 gene - 1082, - 819 and - 592 position polymorphisms were genotyped by amplification refractory mutation systems-polymerase chain reaction (ARMS-PCR) methods in 181 patients with cerebral infarction(CI group) and 115 healthy subjects (control group). Results There was statistical significance in the distribution frequency of IL-10-1082G/A allele in the CI group as compared with the control group (χ~2 = 6.643 ,P=0.010).The IL-10-1082 A allele frequency was 92.3%,and the control group was 86.1%;there was statistical significance between the two groups(P=0.015).There were no statistical significance in genotype distribution frequency of IL-10-592C/A and -819C/T and allele frequency as compared with the control group (P>0.05).Conclusion IL-10-1082G/A gene polymorphisms have been involved in CI,in which the increased A allele frequency is associated with the occurrence of CI.更多还原