【作者】 李凌青；

【导师】 于军；

【作者基本信息】 华中科技大学 ， 微电子学与固体电子学， 2008， 硕士

【摘要】 聚合酶链式反应(Polymerase Chain Reaction, PCR)广泛应用于生命科学的各个领域。集成化的PCR生物芯片充分利用芯片热容量小、比表面积大、节省珍贵样品的优点使得该项技术应用更加方便快捷、高效可靠。本文的研究内容包括:首先针对PCR反应所需的温度均衡性,应用有限元分析方法优化加热电极形状及微腔反应室的高度。采用基于微加工技术的研究方法,在0.2mm厚的玻璃基片集成Pt加热电极及温度传感器,针对高分子聚合物聚碳酸酯(PC)热模压工艺参数进行了研究并制作出芯片微反应室的腔体。其次对于Pt传感器温度电阻公式进行分析测得所制作的Pt传感器的电阻温度系数。之后对传感器微信号的获取进行了深入研究并采用了以精准恒流源LM336-2.5、AD620以及OP07为基础的高精度放大电路。最后采用以FPGA为核心的控制系统,用VHDL硬件描述语言实现包括AMP、AD、PID、PWM和LCD等模块,用ISE软件完成编译下载实现系统功能。该系统有利于PCR系统的升级并能有效集成到微全分析系统中。基于玻璃基片和聚碳酸酯实现的PCR芯片集成了DNA反应室、Pt加热电极及温度传感器等功能组件。微腔反应室容积约为5微升。通过简化芯片的微结构、微加工工艺和微加工设备,制作出较低成本PCR芯片。同时对芯片的控制系统进行了研究,构建了以FPGA为核心的控制系统。更多还原

【Abstract】 Polymerase Chain Reaction (PCR) is widely used in every field of life sciences. Integrated PCR biochip has the advantages of small heat capacity, high specific surface areas and saving Precious samples, which made its application more convenient, faster, efficient and reliable.The research contents of this thesis focus on the temperature proportionality of PCR reaction, to optimize heating electrode shape and the height of micro-cavity Chamber by Finite element analysis. Using Micro-processing technology, Platinum electrode and temperature sensor is integrated into Glass Substrate of 0.2mm. What’s more, polycarbonate hot molding process Parameters is studied and the biochip chamber is made. And then we got Temperature Coefficient of Resistance of platinum sensor that has been made by analyzing Temperature resistance formula of platinum sensor. Later on signal acquisition was studied by applying Amplifying Circuit of High Precision of Precision current source focus on LM336-2.5, AD620 and OP07. Finally control system focused on FPGA was applied; using VHDL Hardware Description Language to implement AMP, AD, PID, PWM, LCD and so on, Compiling and downloading was finished by a software named ISE so as to implement system function. The system can help PCR to upgrade and integrate into Micro-Total Analysis System efficiently.The PCR biochip on Glass Substrate and polycarbonate (PC) integrated into functional unit such as DNA chamber, platinum electrode temperature sensor, etc. The Volume of micro-cavity Chamber is about 5 ul. We can lower the PCR biochip cost by simplifying the micro-structure of biochip, Micro-machining process and equipments. Meanwhile the biochip control system was studied and constructed on FPGA .更多还原