【作者】 王现国；

【导师】 赵金平； 潘铁成；

【作者基本信息】 华中科技大学 ， 外科学， 2008， 硕士

【摘要】 目的探讨落新妇甙对大鼠移植主动脉平滑肌细胞的影响。材料和方法成年SD大鼠30只为供体, Wistar大鼠30只为受体,随机分为2组,落新妇甙组和对照组,每组15只。落新妇甙组:于移植术后当日起采用腹腔注射注射落新妇甙,剂量为5mg/kg.d,连续7天。对照组:术后未接受落新妇甙治疗。各组大鼠于移植术后7,14,28天分批处死,每组每个时间点5只。测定Bcl-2,Bax的表达,行标本组织病理学观察和图象分析。结果与对照组相比,落新妇甙组术后Bax升高( P < 0.05),Bcl-2无明显改变.病理观察显示:落新妇甙组术后各时间段病理损害程度均明显轻于对照组,血管平滑肌细胞增殖明显受到抑制。结论主动脉移植术后早期应用落新妇甙可有效抑制同种大鼠移植动脉硬化,作用机制与抑制血管平滑肌细胞增殖有关。更多还原

【Abstract】 Objective: To evaluate the effect of Astilbin treatment in rat aortic homograft smooth muscle cell.Materials and Methods: Rat AVHs were heterotopically allografted onto abdominal aorta from SD rats (n=30) to Wistar rats (n=30). Wistar rats were divided into two groups, Control group and Astilbin group,15 rats each group.Astilbin group were treated with Astilbin(5mg/kg.d.)respectively within 1 weeks after transplantation. Control group were given no Astilbin. 5 rats were sacrificed in batches at 7、14、28 days postoperatively each group. Aortic homograft specimens were obtained for assessing Bcl-2, Bax, histological studies and image analysis.Results: Compared with controls Bax was significantly increased in Astilbin group. Bcl-2 was no obvious change in tow groups. Histological studies showed: In each time the pathological damage in Astilbin group were significantly lighter than the control group, significantly inhibited proliferation of vascular smooth muscle cell.Conclusion: Astilbin treatment at early stage after transplantation may inhibit arterioscleriosis in aortic allograft, the mechanism is related to inhibition of vascular smooth muscle cell proliferation.更多还原