【作者】 吴艺；

【导师】 尹洪滨； 孙中武；

【作者基本信息】 东北林业大学 ， 细胞生物学， 2008， 硕士

【摘要】 鲟鱼是优质名贵鱼种,具有重要的科研经济价值。本研究通过同工酶分析方法系统研究史氏鲟、西伯利亚鲟、史氏鲟(♀)×西伯利亚鲟(♂)杂交种及史氏鲟(♂)×西伯利亚鲟(♀)杂交种4个鲟鱼群体的遗传多样性,为鲟鱼种质保护、资源开发利用及良种选育与推广提供科学依据。史氏鲟、西伯利亚鲟、史氏鲟(♀)×西伯利亚鲟(♂)杂交种及史氏鲟(♂)×西伯利亚鲟(♀)杂交种的同工酶分析结果表明:1.4个鲟鱼群体的心、肝、肌、眼、肾5种组织间的同工酶表达呈现明显的特异性,其中以EST、α-Amy、ADH、GDH、MDH较为显著。2.通过4个群体5种组织9种同工酶比较分析发现,杂交种的酶带表达比亲本复杂,但多与母本酶带表达相近。3.群体遗传多样性分析表明,4个鲟鱼群体的多态座位比例差异较大,平均值为30.00%,平均每个位点的有效等位基因数为1.3130,平均杂合度预期值为0.1637,各群体的Shannon多样性指数平均为0.2320。Hardy-Weinberg遗传平衡符合度检验表明4个鲟鱼群体遗传变异性比较高,处于杂合子过剩状态。4.聚类分析表明,史氏鲟、史氏鲟(♀)×西伯利亚鲟(♂)杂交种聚为一个分支,西伯利亚鲟、史氏鲟(♂)×西伯利亚鲟(♀)杂交种聚为另一分支,杂交种的遗传信息表达多与母本接近。更多还原

【Abstract】 Acipenser is a high quality valuable fish.Study on the genetic diversity of Acipenser schrenckii、Acipenser baeri、Acipenser schrenckii(♀)×Acipenser baeri(♂)、Acipenser schrenckii(♂)×Acipenser baeri(♀)using allozyme analyses,has important content,at the same time,protects fish genetic diversity and germ plasm resources,and promotes sustainable utility of bioresources.Biochemical genetic differences of Acipenser schrenckii、Acipenser schrenckii(♀)×Acipenser baeri(♂)、Acipenser baeri、Acipenser schrenckii(♂)×Acipenser baeri(♀)were analyzed by polyacrylamide gel electrophoresis.The results indicated:1.the expression of all the isozymes studied was highly tissue-specific.Especially among EST,α-Amy,ADH,GDH,MDH were more apparently.2.Acipenser schrenckii、Acipenser schrenckii(♀)×Acipenser baeri(♂)、Acipenser schrenckii(♂)×Acipenser baeri(♀)、Acipenser baeriwere analyzed by polyacrylamide gel electrophoresis.The results indicated:Acipenser schrenckii(♀)×Acipenser baeri(♂)、Acipenser schrenckii(♂)×Acipenser baeri(♀)showed great difference between Acipenser schrenckii、Acipenser baeri.But close to female parents.3.The percentage of polymorphic loci had small difference among populations,mean value was 30.00%,the average effective number of alleles was 1.3130,the Nei’s expected heterozygosity was0.1637,and Shannon’s Information index was 0.2320.Hardy-Weinberg heredity equilibrium proof-test indicated that Hemibarbus labeo resource quality is good.4.Phylogenetic tree showed Acipenser schrenckii、Acipenser schrenckii(♀)×Acipenser baeri(♂)belong to the same clade,and Acipenser baeri、Acipenser schrenckii{♂)×Acipenser baeri(♀)belong to the same clade.Genetic diversity of their reciprocal close to female parents.更多还原