【作者】 魏红；

【导师】 张开明；

【作者基本信息】 山西医科大学 ， 皮肤病与性病学， 2009， 硕士

【摘要】 背景:银屑病是以表皮过度增殖为主要发病特征的慢性复发性炎症性皮肤病,其根本发病机制尚未完全阐明。一般认为自身反应性T细胞介导的免疫反应为其主要发生机制,而近年来大量国内外研究已证明凋亡在银屑病发病中起着很重要的作用,其特征性的病理改变不仅与角质形成细胞(KC)异常增殖有关,也与KC凋亡异常有关。银屑病表皮角质形成细胞凋亡异常,许多凋亡相关分子的异常表达与其有关。目的:通过检测银屑病与正常人表皮桥粒芯糖蛋白1(Dsg1)的表达,进一步探讨Dsg1在银屑病皮损角质形成细胞凋亡中的作用。方法:采集34例皮肤标本(寻常型银屑病与正常人各17例),用中性蛋白酶分离真表皮,采用逆转录聚合酶链反应(RT-PCR)方法检测寻常型银屑病患者和正常人表皮细胞天冬氨酸特异性半胱氨酸蛋白酶3(caspase-3)与Dsg1mRNA的表达。结果:(1)寻常型银屑病皮损表皮Dsg1mRNA的表达明显高于正常人(0.74±0.12～0.54±0.10)(P<0.05);(2) caspase-3 mRNA在两者间的表达无统计学意义(0.36±0.08～0.39±0.08)(P>0.05)。结论:银屑病患者皮损表皮角质形成细胞的凋亡异常可能与Dsg1的异常表达有关。更多还原

【Abstract】 Background:Psoriasis is a chronic, recurrent inflammatory skin disease characterized by epidermal hyperproliferation. The underlying pathogenic mechanism is still not completely clarified. Self-active T cell-mediated immune response is thoughted to be the main pathological mechanism of psoriasis.However,in recent years numerous studies have demonstrated that apoptosis plays a key role in the pathogenesis of psoriasis and that aberrant expression of many apoptosis-related molecules is related to be abnormal apoptosis of psoriatic keratinocytes.Objective:To explore the action of desmoglein 1(Dsg1)in apoptosis of lesional psoriatic keratinocytes by examining the expression of dsg1 in the psoriatic and the normal epidermis.Methods:A total of 34 skin samples (Biopsies of 17 patients with psoriasis vulgaris and 17 normal skin) were treated by dispase for separating intact epidermis from the dermis.Then the expressions of Dsg1 and caspase-3 in the epidemis were examined by RT-PCR.Results:The psoriatic epidermis showed higher expression of dsg1 mRNA when compared to normal epidemis (0.74±0.12～0.54±0.10) (P < 0.05). There was no significant difference at caspase-3 mRNA level between the psoriatic and the normal epidermis(0.36±0.08～0.39±0.08) (P > 0.05)Conclusion:The results of the study suggested that abnomal apoptosis process of psoriatic epidermal keratinocytes might be related to the aberrant expression of Dsg1.更多还原