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弱活力精子AKAP82mRNA的表达及意义
【作者】 赵双生；
【导师】 徐计秀；
【作者基本信息】 山西医科大学 ， 泌尿外科学， 2009， 硕士
【摘要】 目的:通过测定A激酶锚定蛋白AKAP82mRNA在弱精子症患者及健康成年男性精子中的差异,了解其AKAP82mRNA是否存在异常,探讨其在弱精子症发病机理中可能的作用。方法:采用计算机辅助精液分析系统检测59例弱精子症患者(实验组)和28例健康成年男性(对照组)精液中精子的运动参数,用ELISA方法检测实验组患者血清抗精子抗体(AsAb)。使用低渗肿胀(HOS)试验对两组精子进行分析,通过RT-PCR方法对两组精子中AKAP82mRNA进行检测,实验组患者经提高精子活力活率、抗体专阴治疗后再用RT-PCR方法对其精子AKAP82mRNA进行检测和分析。结果:①59例实验组(17例AsAb阳性,42例AsAb阴性)中有15例标本在RT-PCR检测中有发现有AKAP82mRNA表达缺失,28例对照组中发现2例有AKAP82mRNA表达缺失。有统计学意义(P＜0.05)。其中AsAb阳性组中有4例AKAP82mRNA表达缺失;AsAb阴性组中有11例AKAP82mRNA表达阴性,并无显著性差异(P＞0.05)。②低渗肿胀(HOS)试验中实验组精子尾部总肿胀率,g型精子率均低于对照组(P＜0.05);实验组中15例RT-PCR检测AKAP82mRNA缺失标本精子尾部总肿胀率,g型精子率低于实验组中AKAP82mRNA未缺失标本(P＜0.05)。③15例AKAP82mRNA缺失的患者经提高精子活力活率、抗体专阴治疗后RT-PCR方法检测发现AKAP82mRNA仍然缺失。结论:弱精子症患者AKAP82mRNA表达缺失高于健康成年男性;AsAb阳性患者与阴性组AKAP82mRNA表达无显著差异;弱精子症患者低渗肿胀(HOS)试验精子尾部总肿胀率g型精子率低于健康成年男性;在弱精子症患者中AKAP82mRNA表达缺失患者HOS精子尾部总肿胀率g型精子率低于AKAP82mRNA表达阳性患者。部分弱精子症患者精子的AKAP82异常可能是精子活力低下的原因之一。AKAP82mRNA缺失是造成AKAP82异常表达的原因。
【Abstract】 Objective:AKAP82(A-kinase anchor proteins82) is one of A-kinase anchor proteins in sperm.In order to know the AKAP82mRNA levels of the patients with asthenospermia,we examinated the expressions of AKAP82mRNA in the patients and the healthy fertile men.Methods:Sperm samples were obtained from 59 patients with asthenospermia (experimental group),and the normal sperms were obtained from 28 healthy fertile men(control group).And we estimated motility parameters with a computer-assisted semen analysis(CASA) technique.AsAb(antisperm antibody) of blood serum in the experimental group’s was measured by ELISA(enzyme-linked immunosorbentassay). We measured the two groups AKAP82mRNA by reverse transcriptase PCR.At last, after the experimental group was treated,we measured the levels of AKAP82mRNA by reverse transcriptase PCR.Results:Among the 59 patients,15 patients had been found with AKAP82mRNA microdeletion,and among the 28 healthy fertile men,2 had been found with AKAP82mRNA microdeletion,the percentage of g type sperm and the total tail swelling rate were significantly different(P＜0.05) between control group and experimental group.Conclusion:Abnormality of AKAP82 expression in sperm may be one of the etiological factors in asthenospermia.Abnormality of AKAP82mRNA results in the abnormality of AKAP82 expression.