【作者】 刘辉贤；

【导师】 王天星；

【作者基本信息】 广州中医药大学 ， 中西医结合临床， 2009， 硕士

【摘要】 目的:探讨中医利胆健脾法为主预防微创保胆取石术(MC)后胆囊结石复发以及对豚鼠胆囊收缩素受体(CCK-AR)的机制。方法:临床研究中,将微创保胆取石术(MC)后患者73例,随机分为对照组34例,中药组34例。中药组术后第4天服用利胆健脾法为主方药,对照组未进行任何治疗。术后1年统计疗效(胆囊壁厚度、胆囊收缩率),进行比较;实验研究中,将90只豚鼠随机分为空白组,模型组和治疗组,每组30只,分别喂食标准饲料、致石饲料、致石饲料加防石胶囊,2个月后处死取材,用RT-PCR法检测胆囊壁CCK-AR mRNA表达。同时观察胆囊收缩率及结石率。结果:中药组胆囊壁厚度变薄,胆囊收缩功能改善,且均优于对照组(P<0.05);与模型组比较,治疗组豚鼠胆囊收缩率、CCK-AR mRNA表达增强(P<0.05):结论:利胆健脾法为主方药具有预防微创保胆取石术后结石复发的作用,其作用机制可能与调节胆囊壁CCK-AR mRNA表达有关。更多还原

【Abstract】 Objective To discuss the methods of Cholagogic and Jianpi in the Prevention of Gallstone Recurrence after Stone Removement and this methods in mechanisms of CCK-A receptor of guinea pig.Method:In the part of clinical research,73 cases will be selected after minicholecy-stolithotomyremaning gallbladder patients were randomly divided into control group(34),Fangshi capsule group(34).The control group did not have any treatment after stone removement,Fangshi capsule group in the forth days after removal of the stones began taking Fangshi capsule,All patients observed for aperiod of 1 years(gallbladder wall and gallbladder contraction rate);In the part of Experimental study,90 guinea pig were randomly divided into control group(fed with normal diet,n=30),model group(fed with lithogenic diet,n=30) and treatment group(fed with lithogenic diet plus Fangshi capsule,n=30).After two-months feeding,the animals were sacrificed and sampled,The expression of CCK-A receptor mRNA in gallbladder tissue was subjected to RT-PCR assay,At the sametime, gallbladder contraction rate and the rates of gallstone formation in each group were observed.Results:Compared with the control group,Gallbladder wall of the Fangshi capsule group turned thin,the gallbladder contraction rate were improvement,(P＜0.05);Compared with the model group,increase of gallbladder contraction rate and the expression of CCK-A receptor mRNA (P＜0.05) were found in the treated group.Conclusion:Cholagogic and Jianpi can and prevent the recurrence of gallstone after mini-cholecysto-lithotomyremaning gallbladder,its mechanism is possibly correlated with Regulation of CCK-A receptor mRNA in gallbladder tissue.更多还原