【作者】 郑迪；

【导师】 段舜山；

【作者基本信息】 暨南大学 ， 水生生物学， 2009， 硕士

【摘要】 普通小球藻(Chlorella vulgaris)属于绿藻门(Chlorophyta),绿藻纲(Chlorophyceae),绿球藻目(Chlorococcales),卵囊藻科(Oocystaceae),小球藻属。雨生血球藻(Haematococcuspluvialis)属于绿藻门(Chlorophyta),绿藻纲(Chlorophyceae),团藻目(Volvocales),红球藻科(Haematococceae),红球藻属。鉴于它们富含蛋白质、脂质、多糖和维生素等活性产物,因而成为食品、饵料、保健品、药品、和化妆品等行业竞相研究与开发的优识目标藻种。论文以普通小球藻和雨生血球藻为试验材料,进行了普通小球藻(C)和雨生血球藻(H)5种接种比例(9C:1H、7C:3H、5C:5H、3C:7H和1C:9H),4种氮、磷营养水平和添加方式,以及两种藻间化感效应等三部分实验,研究了普通小球藻与雨生血球藻在不同营养水平下的种间共培养生长效应问题。结果表明:(1)普通小球藻与雨生血球藻以适当接种比例的种间混合培养可以显著提高混合群体产量。4种N、P营养条件下,7C:3H和5C:5H两组处理下的混合藻细胞密度,生物量和叶绿素α都高于纯培养和其它接种比例处理。(2)N、P营养浓度和添加方式对共培养的普通小球藻和雨生血球藻的生长具有显著影响。补充添加N、P营养条件下两种藻的细胞密度、生物量和叶绿素α含量表现出显著促进现象,5C:5H处理的藻细胞密度比正常营养条件高46.3%,9C:1H处理的生物量和叶绿素α含量分别比正常营养条件高77.7%和58.4%。(3)普通小球藻与雨生血球藻混合培养下N、P营养的消耗量普遍高于纯培养处理。至共培养生长末期时,4种N、P营养条件下5C:5H处理的N、P营养消耗量最高,分别为92.7%-99.7%和98.8%-99.4%。(4)普通小球藻与雨生血球藻之间存在化感作用现象。普通小球藻滤液对雨生血球藻的生长具有较明显的化感促进效应,细胞密度的促进量为2.3%-15.8%。雨生血球藻滤液对普通小球藻在正常营养条件下存在化感促进效应,细胞密度的促进量为2.0%-16.8%;在限制营养条件下存在抑制效应,细胞密度的抑制率为10.0%-14.1%。更多还原

【Abstract】 Chlorella vulgaris belongs to Chlorophyta,Chlorophyceae,Chlorococcales,Oocystaceae, Chlorella.Haematococcus pluvialis belongs to Chlorophyta,Chlorophyceae,Volvocales, Haematococceae,Haematococcus.As these two economic algae are rich in proteins,lipids, polysaccharides,vitamins and activity of metabolites,they have wide application prospect and scientific research value in food,cosmetics,health products and pharmaceutical industry and other fields.The experiments were carried out into co-culture Chlorella vulgaris(C) and Haematococcus pluvialis(H) under 5 inoculative proportions(9C:1H、7C:3H、5C:5H、3C:7H、1C:9H),4 different N、P nutrient co-cultivation and allelopathic effect experiments.The results showed(1) Appropriate inoculation ratios between Chlorella vulgaris and Haematococcus pluvialis could significantly improve the growth of co-cultured system.The treatments of 7C:3H and 5C:5H apparently showed algae promotion,which cell density,biomass and chlorophyll a were much more higher than other treatments and controls.(2) N、P nutrition and its adding method had significant impact on the growth of Chlorella vulgaris and Haematococcus pluvialis. In 4 different nutrition experiment,supplemental nutrition showed the highest cell density, biomass and chlorophyll a content,especially its treatment of 5C:5H,which result of cell density were 46.3%higher than that of nomal nutrition,and biomass and chl a of 9C:1H was 77.7%and 58.4%much higher than those of nomal nutrition.(3) N、P nutrient utilization in treatment of co-culture were much higher than mono-culture.At the end period of cell growth,the treatments of 5C:5H had the highest N、P nutrient utilization,92.7%-99.7%and 98.8%-99.4%respectively. (4) There were allelopathic effect between Chlorella vulgaris and Haematococcus pluvialis. Chlorella vulgaris(donor) had promotion effect on Haematococcus pluvialis(acceptor),which growth of cell denstiy was 2.3%-15.8%.Under nomal nutrition,filtrate from Haematococcus pluvialis had promotion effect on Chlorella vulgaris,which growth of cell density was 2.0%-16.8%;however,Under limited nutrition filtrate from Haematococcus pluvialis had inhibition effect on Chlorella vulgaris,and the inhibitory ratio was 10.0%-14.1%.更多还原