【作者】 薛玮；

【导师】 高云芳；

【作者基本信息】 西北大学 ， 动物学， 2009， 硕士

【摘要】 目的:1.研究不同季节达乌尔黄鼠比目鱼肌的肌纤维形态与肌纤维类型的变化。2.研究不同季节尾部悬吊对达乌尔黄鼠比目鱼肌肌纤维形态与肌纤维类型的影响。3.研究冬眠不同时期达乌尔黄鼠比目鱼肌肌纤维形态与肌纤维类型的变化。4.研究冬眠不同时期,达乌尔黄鼠骨骼肌肌红蛋白含量的变化。方法:(1)以尾部悬吊法建立达乌尔黄鼠骨骼肌废用模型。(2)采用免疫组织化学技术,检测达乌尔黄鼠比目鱼肌梭外肌对单克隆抗体MY-32的免疫反应性。(3)彩色图像分析仪测量达乌尔黄鼠骨骼肌比目鱼肌肌纤维横截面积(CSA)的变化。(4)采用紫外分光光度计测定冬眠不同时期达乌尔黄鼠骨骼肌肌红蛋白的含量。结果:1)达乌尔黄鼠比目鱼肌肌纤维形态结构和类型的季节性变化在不同季节中,黄鼠比目鱼肌肌肉湿重在夏季最大,与秋季,冬季冬眠,春季相比有显著性差异(P<0.05),而比目鱼肌肌肉湿重体重比则无显著性差异(P>0.05);夏季比目鱼肌Ⅰ、Ⅱ型肌纤维横截面积分别为2219.66±μm~2和2408.52±μm~2,秋季、冬眠Ⅰ型肌纤维横截面积分别降低了7.7%(P>0.05)、30,01%(P<0.05),而春季反而增大了3.02%(P>0.05),Ⅱ型肌纤维横截面积在秋季、冬眠分别下降了24.29%(P<0.05),28.45%(P<0.05),但春季增大了4.98%(P>0.05);Ⅰ、Ⅱ型肌纤维比例在不同季节中无变化(P>0.05),仅在冬眠中出现了Ⅰ向Ⅱ型的略微转化。2)不同季节尾部悬吊达鸟尔黄鼠对比目鱼肌的影响不同季节尾部悬吊达乌尔黄鼠14天后,黄鼠体重在夏季、冬季和春季分别比对照组减少了2.87%(P>0.05),9.54%(P<0.05),7.67%(P<0.05);比目鱼肌肌肉湿重体重比在夏季、冬季和春季分别比对照组降低了40.83%,29.44%,28.99%,且均具有极其显著性差异(P<0.01);夏季、冬季和春季吊尾组的比目鱼肌肌纤维平均横截面积分别比对照组减少了50.42%,36.91%,24.04%,且均具有极其显著的差异(P<0.01);不同季节吊尾组的比目鱼肌中Ⅰ与Ⅱ型肌纤维类型的构成比均未发生明显变化(P>0.05)。3)达乌尔黄鼠在冬眠不同时期比目鱼肌形态结构与肌纤维类型的变化黄鼠体重在冬眠一个月、冬眠两个月和出眠2天相比冬眠前分别降低了16.32%,21.34%,29.73%;与冬眠前黄鼠相比,冬眠一个月、冬眠两个月和出眠两天的黄鼠皮脂厚度分别下降了11.79%(P>0.05),16.04%(P>0.05),32.55%(P<0.05);冬眠一个月、两个月、出眠两天黄鼠肥胖指数相比冬眠前分别下降了7.12%(P>0.05),12.82%(P<0.05),24.67%(P<0.05);与冬眠前黄鼠相比,冬眠一个月、冬眠两个月黄鼠肌肉湿重体重比分别减少了17.09%(P<0.05),22.22%(P<0.05),而出眠2天黄鼠相比冬眠前黄鼠则无变化:冬眠两个月、出眠2天黄鼠比目鱼肌Ⅰ、Ⅱ肌纤维平均横截面积(1572.4±μm~2)相比冬眠前、冬眠一个月平均肌纤维横截面积(1940.5±μm~2)显著减小(P<0.05);冬眠期间黄鼠比目鱼肌肌纤维比例未发生变化,仅在冬眠两个月时Ⅰ型肌纤维比例有略微增高。4)冬眠不同时期达乌尔黄鼠骨骼肌肌红蛋白含量的变化与冬眠前黄鼠比目鱼肌肌红蛋白含量相比,冬眠一个月、冬眠两个月分别增加了30.67%(P<0.05),31.57%(P<0.05),出眠2天、出眠14天黄鼠与冬眠前黄鼠相比,分别增加了54.66%(P<0.01,),88.86%(P<0.01);出眠14天黄鼠与冬眠一个月、冬眠两个月黄鼠相比,显著增加(P<0.01);冬眠前、冬眠一个月、冬眠两个月、以及出眠2天和出眠14天黄鼠趾长伸肌肌红蛋白含量均无显著性变化(P>0.05)。更多还原

【Abstract】 Objective:1) To study the changes of the morphological structure and the type of myofibrilla in soleus in different seasons. 2) To study the changes of the morphological structure and the type of myofibrilla in soleus in different seasons after tail-suspend 14 days. 3) To study the changes of the morphological structure and the type of myofibrilla in soleus during the different peirod of hibernation. 4) To study the changes of the content of myoglobin in different peirod of hibernation.Methods:1) Establish the disuse model of skeletal muscle was achieved by tail-suspension. 2) Fiber types of solues muscle(SOL) in ground squirrel were identified by immunohistochemistry technology with antibody fast-twitch mysion heavy chain(MHC). 3) The cross-sectional area (CSA) of soleus muscle fiber was measured by image analysis software. 4) The myoglobin content of skeletal muscle was measured by the spectrophotometer.Result:1) The comparison of configuration and muscle fiber types of SOL in normal groundsquirrel in fifferent seasons.The SOL muscle wet weight in summer had significant differences between summer and other seasons. But the ratios of the muscle wet weight to body weight had no differences between different seasons (P>0.05); The CSA of the typeⅠmuscle fiber of soleus of ground squirrel was 2219.66μm~2 ,the CSA of the typeⅡmuscle fiber of soleus was 2408.52μm~2. Compared with summer, the CSA of the typeⅠfiber in autumn and winter decreased 7.7%(P>0.05) and 30.01%(P<0.05), respectively, but the spring increased about 3.02%(P>0.05). The CSA of the typeⅡfiber in autumn and winter decreased 24.29%(P<0.05) and 28.45%(P<0.05) , respectively, but the spring increased about 4.98% (P>0.05). The percentage of muscle fiberⅠandⅡhad no changes in different seasons, but in winter has a little upgread tendency.2) The effection of tail-suspend on configration and muscle fiber type of soleus in ground squirrel in fifferent seasons.After 14 days suspension, the body weight of the Ground Squirrel decreased about 2.87%(P>0.05), 9.54%(P<0.05), 7.67%(P<0.05) compared with the control group in sunmmer, winter and spring. The ratios of the muscle wet weight to the body weight had significant difference compared with the control group. The average CSA of the soleus muscle fibers decreased about 50.42%(P<0.01), 36.91%(P<0.01), 24.04%(P<0.01) in summer, winter and spring group compared with the control group. Compared with the control group(summer, winter, spring), the percentage of the muscle fiber had no changes, the muscle type had not transformed.3) The effection of hibernation on configuration and muscle fiber type in SOL in Ground Squirrel.Compared with the CON Ground Squirrel, the body weight of the H-1mon, H-2mon and AH(2d) decreased 16.32%, 21.34%, 29.73%, respectively. The ratios of the muscle wet weight to body weight showed descent tendency during hibernation, compared with the H-2mon, the ratios of the CON and AH(2d) had significant difference (P<0.05). The sebum cutaneum thickness of the H-1mon, H-2mon and AH(2d) decreased about 11.79%(P>0.05), 16.04%(P>0.05), 32.55%(P<0.05) , respectively , compared with the CON. The index of corpulence of the H-1mon, H-2mon and AH(2d) decreased about 7.12%(.P>0.05), 12.82%(P<0.05), 24.67%(P<0.05) respectively , compared with the CON. The CSA of the muscle fiberⅠandⅡin H-2mon and AH(2d) of the Ground Squirrel had significant difference from the CON and H-1mon(P<0.05).The percentage of typesⅠandⅡfibers in soleus did not show significant variation in ground squirrl during hibernation (P>0.05) but only the H-2mon had a little upgrade tendrncy.4) The myoglobin content of skeletal muscle(SOL, EDL) of the Ground Squirrel during different period of the hibernation. Compared with the CON group, the content of myoglobin in H-1mon and H-2mon group had a significant difference(P＜0.05), and had a extremely significant difference with AH(2d) and AH(14d) group(P<0.01). The AH(14d) group compared with the hibernation group also had a extremely significant difference(P<0.01). The EDL myoglobin content had no changes in any group(P>0.05).更多还原