【作者】 蒋德付；

【导师】 张小里；

【作者基本信息】 西北大学 ， 化学工程， 2009， 硕士

【摘要】 高纯度磷脂酰乙醇胺(PE)在提高人体记忆力与增强大脑功能方面优于磷脂酰胆碱(PC),磷脂酰乙醇胺具有维持及改善认知功能,其应用价值极其为重要;PE在天然磷脂组成中含量较低,这使得对其纯化较为困难;在天然磷脂酰乙醇胺的应用过程中,由于脂肪链的不饱和性,使得其化学稳定性、分散性、乳化性等都不好,而通过合成具有饱和脂肪链的磷脂酰乙醇胺,其用途更广、更安全,尤其是在医药和食品工业中。目前国内所用高纯度磷脂产品多从国外进口,年需求量为数吨以上,而传统研究中又以PC居多,忽略了PE的重要性能,所以本实验的主要目的是研究获得高纯度PE的方法,同时对天然磷脂进行改性,提高PE在天然磷脂中的含量,并得到结构稳定的磷脂酰乙醇胺,经柱层析分离后能达到医药和食品等领域应用的纯度要求。本文以天然磷脂PC-60为原料,运用硅胶柱层析法,考察天然磷脂在硅胶柱中的洗脱情况,在最佳工艺条件下,即氯仿/甲醇=2/3(v/v),室温下操作,上样量为0.025g磷脂粗品/g硅胶,流速控制在3ml/min左右,可得到纯度为96.2%,回收率为89.6%的磷脂酰乙醇胺:通过酶转化法使得磷脂酰乙醇胺在天然磷脂中的含量提高到80%左右,对酶转化后合成的磷脂酰乙醇胺进行分离纯化,在最佳洗脱工艺下,得到纯度为98.3%,回收率达90.4%的磷脂酰乙醇胺,能满足高纯度磷脂制备的要求,同时还选用了其它低毒的洗脱剂,也得到了不错的效果。本实验还用硬脂酸与天然磷脂中的不饱和链进行脂肪酰置换反应,经柱分离得到纯度高于95%的二硬脂酰磷脂酰乙醇胺产品,红外分析表明,没有不饱和键的吸收峰,这丰富了对磷脂酰乙醇胺的研究,为日后磷脂酰乙醇胺的工业化生产提供了可靠的数据支持。更多还原

【Abstract】 The high-purity phosphatidylethanolamine (PE) surpasses phosphatidylcholine (PC) regarding improving human body memory and enhancing cerebrum function. Simultaneously, the high-purity PE has the function of maintaining and improving cognition. Its application value is extremely important. The content of PE in natural phospholipids is low, so its separation and purification are difficult. In the application of lecithin, as a result of instauration of the fat chain, its chemical stability, dispersivity, emulsibility and so on are not good. But the application of synthetical lecithin having the saturated fat chain is broader and safer, particularly in medicine and food industry.At present, the most high-purity phospholipids products used in domestic mostly depends on import from overseas. The demand of high-purity lecithin reaches several tons per year. But the traditional research focuses on PC, neglecting importance of PE. Therefore, the main purpose of this experiment is to obtain the high-purity PE method, simultaneously carry on the modification to the molecular structure of PE to obtain stable structure, which can meet the needs of PE in medicine, food industries and so on.This article takes natural phospholipids PC-60 as raw material, using the silica gel column chromatography and isocratic elution, to inspect elution situation of phospholipids in the silica gel column. The best technological condition is that the eluent is chloroform/methyl alcohol (2/3, v/v), under the room temperature, loading amount is 0.025g phospholipids raw material /g silica gel, and the speed of flow is about 3ml/min. Under above conditions, the purity and recovery percent of PE can reach 96.2% and 89.6%. By enzymatic conversion method, the content of PE in natural phospholipids can be raised to 80%. In the separation process of synthesis PE obtained by enzymatic conversion method, the best technological conditions is that the eluent is chloroform/methyl alcohol (1/2, v/v), under the room temperature, loading amount is 0.03g phospholipids raw material /g silica gel, the speed of flow is about 45 drop /min. Under above conditions, the purity of PE can reach 98.3%, the recovery percent is 90.4%, which can satisfy the request of high-purity phospholipids preparation. Meanwhile other low poisonous eluents have been selected, also obtaining the good result.This experiment also carries on replacement reaction between the stearic acid and the unsaturated chain of natural phospholipids, obtaining Distearoyl-Phosphatidylethanolamine (DSPE) whose purity is higher than 95% by column separation. The infrared analysis indicated that DSPE hasn’t absorption peak of unsaturated bond, which has enriched the PE research, and provided reliable data support for the industrialization of PE production in the future.更多还原