加拿大杨树芽化学成分的研究

【作者】 王想想；

【导师】 周立东；

【作者基本信息】 中国协和医科大学 ， 生药学， 2008， 硕士

【摘要】 对加拿大杨树芽化学成分进行了基础性研究,该研究对揭示北温带蜂胶与杨树芽化学成分的差异,以及西蜂采集树芽形成蜂胶的过程中的生物转化过程具有重要意义,有助于进一步开展蜂胶的质量控制和相关研究。本文对加拿大杨的化学成分进行了系统研究。利用硅胶柱色谱,制备TLC,聚酰胺柱色谱,大孔树脂和Sephadex LH-20柱色谱等手段,从加杨芽中分离得到15个化合物,其中黄酮苷元8个,黄酮苷3个,有机酸2个,其它酚苷2个。根据理化性质和波谱数据并与文献对照,分别鉴定为:球松素(Pinostrobin,Ⅰ)、白杨素(Chrysin,Ⅱ)、山柰酚(Kaempferol,Ⅲ)、芹菜素(Apigenin,Ⅳ)、异鼠李素(Isorhamnetin,Ⅴ)、异阿魏酸(Isoferulic acid,Ⅵ)、槲皮素(Quercetin,Ⅶ)、3-O-甲基槲皮素(3-O-methyl quercetin,Ⅷ)、咖啡酸(Caffeic aeidⅨ)、高良姜素-7-O-β-D-葡萄糖苷(Galangin-7-glucoside,Ⅹ)、白杨素-7-O-β-D-葡萄糖苷(Chrysin-7-glucoside,Ⅺ)、高良姜素(Galangin,Ⅻ)、乔松素-5-O-β-D-葡萄糖苷(Pinocembrin-5-glucoside,ⅩⅢ)、水杨苷(Salicin,ⅩⅣ)、紫丁香苷(Syringin,ⅩⅤ)。化合物Ⅷ、Ⅹ、ⅩⅢ和ⅩⅤ为首次从该属植物中分离得到。本文对蜂胶及其胶源植物化学成分的相关性进行了综述,比较了蜂胶及其胶源植物化学成分的差异。最后,对研究结果进行了讨论。更多还原

【Abstract】 The chemical constituents of Populus canadensis Moench.buds were investigated. The result was important for constituents comparison of north temperate zone propolis and poplar buds extract.It’s helpful for quality control of propolis and chemical relationship research between propolis and it’s plant sources.Fifteen compounds were isolated by chromatographic techniques with different materials,such as silica gel,macroporous resin D101,polyamide gel,sephadex LH-20, etc.Their structures were elucidated on the basis of physicochemical properties, spectral analysis of UV,IR,MS and NMR,and TLC characteristic comparison with standard samples.They were identified asⅠ)pinostrobin;Ⅱ)chrysin;Ⅲ)kaempferol;Ⅳ)apigenin;Ⅴ)isorhamnetin;Ⅵ)isoferulic acid;Ⅶ)quercetin;Ⅷ)3-O-methyl quercetin;Ⅸ)caffeic acid;Ⅹ)galangin-7-glucoside;Ⅺ)chrysin-7-glucoside;Ⅻ) galangin;ⅩⅢ)pinocembrin-5-glucoside;ⅩⅣ)salicin;ⅩⅤ)syringin.The compoundsⅧ,Ⅹ,ⅩⅢandⅩⅤwere isolated from the genus Populus L.for the first time.The advances in research of the chemical relationship between propolis and it’s plant sources were summarized.The results were discussed.更多还原