【作者】 罗科学；

【导师】 潘智敏；

【作者基本信息】 浙江中医药大学 ， 中医内科学， 2008， 硕士

【摘要】 目的采用酒精加高脂乳剂建立大鼠脂肪肝模型,通过观察调脂积冲剂对实验大鼠血清总胆固醇(TC)、血清甘油三脂(TG)、血清高密度脂蛋白(HDL-C)、血清低密度脂蛋白(LDL-C)、空腹血糖(FBG)、血清谷氨酸氨基转肽酶(ALT)、血清谷氨酰转肽酶(GGT)、空腹胰岛素(FINS)、肝组织病理及肝指数等指标的检测,并计算胰岛素抵抗指数(HOMA—IR)=FBG~*FINS/22.5。以此探讨调脂积冲剂防治脂肪肝的有效性、可靠性,为进一步研究提供理论依据。方法将Wistar大鼠52只,于实验室适应性喂养一周后,随机分组共分五组,其中空白对照组、脂肪肝模型对照组、脂肪肝模型易复善治疗组、脂肪肝模型中药调脂积冲剂治疗组、脂肪肝模型中药(六味地黄丸)治疗组。采用酒精合并高脂乳剂建立大鼠脂肪肝模型,以易善复和六味地黄丸为阳性对照,利用全自动生化分析仪中检测TC、TG、HDL-C、LDL-C、ALT、GGT、FBG,采用酶联免疫法测定血清胰岛素含量。胰岛B细胞功能评价采用稳态模式评估法(HOMA),即胰岛素抵抗指数(HOMA—IR)=(FBG)~*FINS/22.5。采用光镜观察组织学变化。结果模型组TC、TG、LDL-C、ALT、GGT、FBG、FINS、HOMA—IR、肝指数均高于正常组,HDL-C低于正常组。调脂积冲剂能降低肝指数,降低ALT、GGT、TC、TG、LDL-C、FBG、FINS的含量,升高HDL-C的含量,降低HOMA—IR。结论调脂积冲剂防治脂肪肝的作用机制:1.调脂积冲剂具有保护肝细胞,稳定和修复细胞膜结构及线粒体等各种肝细胞器的功能,改善肝功能的作用。2.调脂积冲剂可改善血脂代谢紊乱,防治高脂血症的作用。3.调脂积冲剂可改善胰岛B细胞的高分泌状态,降低胰岛素分泌量,提高机体对胰岛素的敏感性,改善胰岛素抵抗。4.调脂积冲剂可降低肝细胞脂肪变性,保护肝组织的作用。更多还原

【Abstract】 Objective To establish a hepatic fibrosis model in rat according to multiple factors, compound factors rat fatty liver model by observing TiaozhiJi Emulsion on experimental plot in TC,TG,HDL-C,LDL-C,FBG,ALT,GGT,FLNS,the liver and liver pathology index Detection of such indicators,and calculate HOMA-IR = FBG * FINS/22.5.TiaozhiJi Emulsion of this fatty liver of the validity,reliability,and insulin resistance in the formation of fatty liver’s role in the process.Methods 52 Wistar male rats,a week after the laboratory feeding adaptation,randomized divided into five groups,the control group,the control group model of fatty liver,fatty liver model of good easy-treatment group,fatty liver Model medicine TiaozhiJi Emulsion treatment group,fatty liver model of traditional Chinese medicine(Liuwei- dihuang pill) in the treatment group.Combined high-fat emulsion used alcohol fatty liver rat model to Essentiale and Liuweidihuangwan to as a positive control,the use of automatic biochemical analyzer detected TC,TG,HDL-C,LDL-C,ALT and GGT,FBG,using enzyme-linked immunosorbent assay for determination of serum insulin levels.B islet cell function evaluation by homeostasis model assessment(HOMA),or insulin resistance index (HOMA-IR) =(FBG) * FINS/22.5.Results The TC,TG,LDL-C,ALT and GGT,FBG,FINS,HOMA-IR,liver index were higher than those of the normal group,lower HDL-C group.TiaozhiJi Emulsion can reduce the liver index,lower ALT and GGT,TC,TG,LDL-C,FBG,FINS content,lower HOMA-IR.Conclusion TiaozhiJi Emulsion control the mechanism of fatty liver:1.TiaozhiJi Emulsion protect liver cells,and restoration of stability and structure of mitochondrial membrane such as the liver cell functions,and improve liver function.2.TiaozhiJi Emulsion can improve blood lipid metabolism disorders,prevention and treatment of hyperlipidemia. 3.TiaozhiJi Emulsion can improve islet B-cell secretion of the high state and decrease insulin secretion,improving the body’s sensitivity to insulin and improve insulin resistance.4.TiaozhiJi Emulsion can reduce rat liver liver index,reduced fat deposition in the liver and improve the role of fatty degeneration of the liver更多还原