【作者】 赵晓丽；

【导师】 肖雯；

【作者基本信息】 甘肃农业大学 ， 植物学， 2008， 硕士

【摘要】 本实验以15种栽培常见的园林绿化树种作为研究对象,在人工熏气室内对其二年生截枝分别进行不同SO2浓度和不同时间熏气处理,测定了在SO2熏气处理下各树种叶片中叶绿素含量(Chl)、细胞汁液pH值、细胞膜相对透性(PMP)、丙二醛(MDA)、游离脯氨酸(Pro)、可溶性糖(SS)和可溶性蛋白(SP)的动态变化,运用相关分析、主成分分析、聚类分析等数学方法对不同树种主要的抗性指标进行了综合评价,系统论述了各树种对SO2的反应特性,进一步探讨了SO2对树木的伤害机理。主要实验结果如下:1.采用不同浓度SO2熏气对15种常见栽培园林绿化树种叶片进行各种生理生化指标的测定,结果表明:大部分树种在SO2胁迫下其叶片生理生化指标都呈现有规律的变化,叶绿素含量在胁迫第一天都有较显著的下降,随胁迫时间的延后稍有回升,叶绿素a/b值、游离脯氨酸含量则有上升有下降,质膜相对透性、可溶性糖含量、可溶性蛋白含量和丙二醛含量总体呈上升趋势,而细胞汁液pH值则下降。这说明大部分树种应对SO2胁迫都产生了相同的生理响应,而少部分树种则可能有它应对胁迫独特的生理调节功能。2.通过叶绿素含量与其它各项指标的相关回归分析表明:大部分树种生理生化指标与叶绿素的相关性并不高,虽然大部分树种的生理生化指标变化都有一定的规律,但是其变化的幅度并不一致,各指标的变化未呈现对应性。在SO2胁迫后,叶片叶绿素总量与脯氨酸含量和可溶性蛋白都呈正相关的是山桃;与丙二醛呈正相关的树种是紫叶小檗;与可溶性糖含量呈极显著正相关的树种是栾树。3.运用主成分分析法对不同树种的生理生化指标进行综合评判表明:各个树种在抗SO2机制中构成因素很多,应根据抗性指标的变化综合考量。各个树种对SO2胁迫的适应性不同,所起主导作用的生理因素也不相同,评判一个树种抗SO2的能力应综合考虑各种生理生化指标的变化,并找出起主导作用的评价指标。SO2胁迫下连翘、榆叶梅、牡丹、栓翅卫矛的叶绿素含量与相对含水量因素得分最高,元宝枫的叶绿素a/b和质膜相对透性因素得分最高,紫丁香、小叶黄杨的丙二醛因素得分最高,栾树细胞汁液pH值与核酸因素得分最高,悬铃木、长叶白蜡的可溶性蛋白因素得分最高,山桃、华北珍珠梅的可溶性糖因素得分最高。4.采用主成分法将SO2胁迫下不同树种的综合因素得分从大到小进行排序,其结果为:榆叶梅﹥栾树﹥连翘﹥紫丁香﹥牡丹﹥元宝枫﹥悬铃木﹥栓翅卫矛﹥华北珍珠梅﹥山桃﹥长叶白蜡﹥小叶黄杨。5.采用聚类分析方法对12个树种进行分类,结果显示:山桃、栓翅卫矛、连翘、悬铃木、小叶黄杨、紫丁香,榆叶梅和华北珍珠梅为一类,牡丹、长叶白蜡和元宝枫为一类,栾树为一类。一类树种在具有某些共同特征的同时,也存在许多指标变动较大的现象,这说明各树种在每一指标上的差别是比较大的,仅靠单一指标将各树种对SO2抗性的强弱进行分类是片面的,必须综合考虑较多的因素才能使评价结果更加准确。更多还原

【Abstract】 Sulfur dioxide is one of the primary pollutant in urban environments, it usually leads to the afforestation plant changes in morphology, structure and physiological metabolism. Two-year-old cutted branches of fifteen ornamental tree species were fumigated by sulfur dioxide at different concentrations level and with different treatments time. The purpose is to test the dynamic changes of chlorophyll(Chl) a, b, t contents, pH of the cellular juice, plasma membrane permeability(PMP) and malondialdhyde(MDA), proline(Pro), soluble sugar(SS), soluble protein(SP) contents in the leaves, and to evaluate the main resistive indexes of tree varieties comprehensively by mathematical methods such as correlation analysis, cluster analysis and principal component analysis. Then systematic discussed each tree specie, reaction to sulfur dioxide and further explored the injury mechanisms of sulfur dioxide. The main results are as follows:1. Physiological and biochemical indexes of 15 kinds of common cultural ornamental tree leaves which fumigated with different SO2 concentrations were determined in this experiment. The results showed that: the physiological and biochemical indexes in leaves of most species all showed a regulated change. Chlorophyll content of most species had a significant descend at first day, and then ascended along with the prolongation of stress time and the increase of the SO2 concentration; the Chla/b and Pro content also went up and down; PMP, SS, SP and MDA content were increased in general, but the cellular juice pH was reduced. It revealed that most species had the same physiological response, while a small number of species may has its unique physical conditioning under SO2 stress.2. By studying the correlation regression analysis between chlorophyll content and each other index, the results showed that: physiological and biochemical indices of most species had a low correlation with chlorophyll content, Although the changes of physiological and biochemical indices of most species had certain laws, the range was inconsistency, and the changes of each index were not shown correspondence. After SO2 treatment, it was Amygdalus davidiana which was positive with chlorophyll content and Pro, SS, and Berberis thunbergii‘Atropurpurea’with chlorophyll and MDA, and Koelreuteria paniculata which had significant positive with SS.3. By comprehensive evaluation the physiological and biochemical indices of different tree species with principal component analysis, the results showed that There were many factors that had effects on the SO2 resistant mechanism, so it should be had a comprehensive evaluation based on the changes of resistant indices. Different tree species had different adaptability, and the magistral physiological factors were also not the same. So it should be comprehensively considered the changes of each physiological and biochemical index, and found out the evaluation index of magistral factors.The Chl content and relative water content of Amygdalus triloba, Forsythia suspensa, Paeonia suffruticosa, Euonymus phellomanus had the highest score under SO2 stress, and the chla/b, PMP of Acer truncatum, the MDA content of Syringa oblata, Buxus microphylla, the celluar juice pH and nucleic acid of Koelreuteria paniculata, the SP content of Platanus orientalis, Fraxinus pennsylvanica var. lanceolata and the SS content score of Amygdalus davidiana, Sorbaria kirilowii also had the highest score.4. Principal component analysis was used to comprehensive evaluation of the resistance of twelve ornamental tree species under SO2 stress. The sulfur dioxide resistance of the twelve tree species from strong to weak is as the followings: Amygdalus triloba﹥Koelreuteria paniculata﹥Forsythia suspensa﹥Syringa oblata﹥Paeonia suffruticosa﹥Acer truncatum﹥Platanus orientalis﹥Euonymus phellomanus﹥Sorbaria kirilowii﹥Amygdalus davidiana﹥Fraxinus pennsylvanica var. lanceolata﹥Buxus microphylla.5. Twelve species was classified by cluster analysis, the results showed that Amygdalus triloba, Sorbaria kirilowii, Amygdalus davidiana, Forsythia suspensa, Syringa oblata, Platanus orientalis, Euonymus phellomanus and Buxus microphylla were the same category; Paeonia suffruticosa, Fraxinus pennsylvanica var. lanceolata and Acer truncatum were a category and Koelreuteria paniculata was another. Every similar species had a certain common characteristics, while there was also a phenomenon that many indices had a larger change. This showed that each species had a large difference on one index, so it was unilateral to sort the SO2 resistant of each tree species only according to one single index, it must be considered more factors to make the results more accurate.更多还原