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艾炷灸膻中、膈俞穴预处理对大鼠心肌缺血—再灌注损伤心肌细胞凋亡基因的影响
【作者】 孟君；
【导师】 李胜涛；
【作者基本信息】 成都中医药大学 ， 针灸推拿学， 2008， 硕士
【摘要】 实验目的:研究艾炷灸膻中、膈俞穴益气活血法预处理对大鼠心肌缺血-再灌注损伤心肌细胞凋亡基因的影响。为临床防治心肌缺血-再灌注损伤提供新的思路和方法。实验方法:通过结扎大鼠左冠状动脉前降支(LAD)造成心肌缺血再灌注模型,取心脏进行心肌病理学观察,免疫组化法测caspase-3、bcl-2、bax、fas、TNF-α及NF-_kB蛋白表达。实验结果:(1)心肌缺血-再灌注损伤后心肌细胞数量减少,细胞直径变小,细胞体积变小。艾炷灸膻中、膈俞穴益气活血法预处理可以使心肌细胞数量增多,细胞直径变大,细胞体积变大。(2)凋亡标志因子caspase-3在心肌缺血再灌注损伤后表达增多,艾炷灸膻中、膈俞穴益气活血法预处理可降低其表达。(3)抑凋亡基因bcl-2在心肌缺血再灌注损伤后表达减少,艾炷灸膻中、膈俞穴益气活血法预处理可使bcl-2表达增多。(4)促凋亡基因bax和fas在心肌缺血再灌注损伤后表达增高,艾炷灸膻中、膈俞穴益气活血法预处理可使bax和fas表达降低。(5)肿瘤坏死因子TNF-α在心肌缺血再灌注损伤后表达增多,艾炷灸膻中、膈俞穴益气活血法预处理可使TNF-α表达减少。(6)诱发凋亡的核转录因子NF-_kB在心肌缺血再灌注损伤后表达增多,艾炷灸膻中、膈俞穴益气活血法预处理可使NF-_kB表达减少。实验结论:艾炷灸膻中、膈俞穴益气活血法预处理对大鼠心肌缺血再灌注损伤起抑制作用。其作用可能通过降低caspase-3表达,增高bcl-2表达,降低bax和fas表达,减少TNF-α和NF-_kB表达来表现。
【Abstract】 Objective:To study the effects of nourishing the Qi and remove the blood pretreatment by moxibustion on myocardial apoptosis induced by ischemia-reperfusion.Methods:The left anterior descene coronary artery was ligated to establish the ischemia-reperfusion heart model in rat.Immunohistochemical staining was used to detect the changes of apoptosis and protein expression of caspase-3、bcl-2、bax、fas、TNF-αand NF-kB.Histopathological changes in myocardium was also observed.Result:（1） The number and size of cardiomyocyte cell reduced after ischemia-reperfusion and increased by nourishing the Qi and remove the blood pretreatment of moxibustion.（2） The expression of caspase-3 gene increased by ischemia-reperfusion and decreased by nourishing the Qi and remove the blood pretreatment of moxibustion.（3） The expression of bcl-2 gene decreased by ischemia - reperfusion and increased by nourishing the Qi and remove the blood pretreatment of moxibustion.（4） The expression of bax and fas gene inregulating after schemia-reper fusion and downregulating after nourishing the Qi and remove the blood pretreatment of moxibustion.（5） The expression of TNF-αand NF-kB increased by ischemiareperfusion and decreased by nourishing the Qi and remove the blood pretreatment of moxibustion.Conclusions:Nourishing the Qi and remove the blood pretreatment by moxibustion had obviously preventive and therapeutic effects on reperfusion injury and could inhibit cardiomyocyte apoptosis.The mechanism might be that bcl-2 gene expression was increased,caspase-3 gene、bax gene、faa gene、TNF-αand NF-kB were decreased following ischemia and reperfusion.