艾炷灸循经取穴预处理对大鼠心肌缺血再灌注损伤心肌细胞凋亡基因的影响

【作者】 郭小燕；

【导师】 李胜涛；

【作者基本信息】 成都中医药大学 ， 针灸推拿学， 2008， 硕士

【摘要】 实验目的:研究艾炷灸循经取穴预处理对大鼠心肌缺血再灌注损伤心肌细胞凋亡基因的影响。实验方法:通过艾炷灸循经取穴预处理2周,然后结扎大鼠左冠状动脉前降支(LAD),造成心肌缺血再灌注损伤模型,取心脏进行心肌病理学观察,免疫组化法检测Caspase-3;Bcl-2;Bax;Fas;TNF-α及NF-κB的表达。实验结果:(1)心肌缺血再灌注损伤后心肌细胞数量减少,细胞直径变小,细胞体积变小。艾炷灸(内关、郄门)预处理可以使心肌细胞数量增多,细胞直径变大,细胞体积变大。(2)凋亡标志因子Caspase-3在心肌缺血再灌注损伤后表达增多,艾炷灸(内关、郄门)预处理可降低其表达。(3)抑凋亡基因Bcl-2在心肌缺血再灌注损伤后表达减少,艾炷灸(内关、郄门)预处理可使Bcl-2表达增多。(4)促凋亡基因Bax,TNF-α,NF-κB与Fas在心肌缺血再灌注损伤后表达增高,艾炷灸(内关、郄门)预处理可使Bax,Fas,TNF-α与NF-κB表达降低。实验结论:艾炷灸循经取穴(内关、郄门)预处理对大鼠心肌缺血再灌注损伤心肌细胞凋亡起抑制作用。其作用可能是通过降低Caspase-3表达,增高Bcl-2表达,降低Bax,Fas,NF-κB与TNF-α表达来表现。更多还原

【Abstract】 Objective:To study the effect of moxbustion on PC4 and PC6 on mycardial apoptosis induced by ischemia-reperfusion(I/R).Methods:The left anterior descene(LAD)coronary was ligate toestablish the ischemia-reperfusion(I/R)heart model in rats.Immunohistochemical staining was to detect the changes of apoptosis and protein expression of Caspase-3,Bcl-2,Bax,Fas, TNF-αand NF-κB.Histopathological changes in myocardium was also observed. Results:(1)The number and size of cardiomycyte cell reduced after ischemia-reperfusion and increased by moxbustion.(2)The expression of caspase-3 gene increased by ischemia-reperfusion and decreased by moxbustion.(3)The expression of Bcl-2 gene decreased by ischemia-reperfusion and increased by moxbustion.(4) The expression of Bax,Fas,TNF-αand NF-κB gene inregulating after ischemia-reperfusion and downregulating after moxbustion.Conclusions:Moxbustion on PC4 and PC6 had obviously preventive and therapeutic effects on reperfusion injury and could inhibit cardiomycyte apoptosis.The mechanism might be that Bcl-2 gene expression was increased,Caspase-3 gene,Bax gene,Fas gene,TNF-αgene and NF-κB gene were decreased following ischemia and reperfusion.更多还原