三拗芎葶合剂对低氧性肺动脉高压大鼠血浆心房利钠肽、C型利钠肽影响的实验研究

【作者】 常生杰；

【导师】 杨仁旭；

【作者基本信息】 成都中医药大学 ， 中医内科学， 2007， 硕士

【摘要】 目的研究中药复方制剂三拗芎葶合剂对低氧性肺动脉高压(HPH)大鼠血浆ANP、CNP的影响,并初步探讨其对HPH肺血管结构重建的防治作用及肺血管活性物质之间的调节作用。方法利用常压缺氧的方法复制低氧性肺动脉高压大鼠模型。雄性SD大鼠50只,随机分为5组:正常对照组、模型组、三拗芎葶合剂中、高剂量组及卡托普利组,每组10只。在造模两周后饲喂相应药物,连续用药28d。采用改良右心导管法测定大鼠血流动力学参数,以放免法测定血浆CNP和ANP水平。处死大鼠后,称量肺湿重wW、右心室自由壁重(RV)和左心室及室间隔重LV+S,计算右心肥厚指数(RV/LV+S)及肺渗出指数(wW/BW);利用图象分析方法,测定肺小动脉病理及其形态计量学。结果(1)三拗芎葶合剂及卡托普利均能明显降低模型大鼠的mPAP、wW/BW、RV/LV+S;三拗芎葶合剂各剂量组与卡托普利组疗效相当。(2)三拗芎葶合剂及卡托普利均能部分逆转模型大鼠的肺血管重构,降低血浆ANP和CNP水平。但治疗组的上述各指标仍未完全恢复到正常对照组水平。结论三拗芎葶合剂能有效降低肺心病模型大鼠的肺动脉高压,部分逆转肺血管结构重建,改善右心功能,可显着降低大鼠血浆C型利钠肽和心钠肽水平。更多还原

【Abstract】 Objective To investigate the effect of SanAoXiongTing combined dosage to big rat’s plasma ANP and CNP that has been suffered from HPH and discuss prevention and curing effect of SanAoXiongTing in HPH induced pulmonary vascular remodeling and modulating function among pulmonary vasoactive substance.Method Utilize the method which is short of oxygen of the atmosphere to duplicate hypoxia big mouse’s model of high pressure of lung artery.50 big mice of male SD,are divided into 5 groups at random:Contrast group,model group,while katuopuli solution group,big-dosage group of Chinese Medicine,mid-dosage group, 10 every groups,respectively irrigating stomach for four weeks with equal quantity of NS,katuopul solution,SanAoXiongTing combined dosage,feeding under the same condition of whole groups,use medicine 28d in succession,consequently examining mPAP,PASP,PADP with the method of right heart vessel;and examining CNP, ANP with the method of Radiation& Immunity.After putting the big mouse to death, weigh the free wall heavy RV and left ventricle and interval heavy LV+S of room of wet heavy wW of lung right ventricle,calculating the plump index(RV/LV+S) of right heart,and the lung ooze out the index(wW/BW);Utilize the analytical method of the vision,determine lung small artery pathology and shape metrology.Result(1)SanAoXiongTing combined dosage and katuopuli solution group can reduce mPAP,wW/BW,RV/LV+S of mouse obviously,SanAoXiongTing combined dosage group is equivalent to to katuopuli solution group.(2)SanAoXiongTing combined dosage group and katuopuli solution group can reverse model lung blood vessel of mouse construct again obviously,reducing CNP,ANP level.But treat every above-mentioned index of the group and does not totally resume to contrasting group’s level normally yet.Conclusion SanAoXiongTing combined dosage can reduce Pulmonary arterial hypertension of model mouse effectively,the lung blood vessel structure is rebuilt to reverse partly,improve the right heart function,and its can reduce plasma CNP,ANP notably.更多还原