附子配伍大黄调控药性物质基础研究

【作者】 叶强；

【导师】 郭力；

【作者基本信息】 成都中医药大学 ， 药物化学， 2008， 硕士

【摘要】 目的:研究附子配伍大黄传统应用环节中各因素(配伍比例、煎煮时间、先下后下)对指标性成分的影响,初步揭示附子大黄寒热配伍调控药性的关键因子和物质基础,为附子配伍大黄变寒下为温下的配伍理论提供科学依据。方法:以附子、大黄为研究对象,运用UV、HPLC、TLCS等现代分析技术,以附子热性主要有效组分附子总生物碱、大黄寒性主要有效组分大黄总蒽醌为指标,通过构建“总生物碱—酯型生物碱—双酯型生物碱(新乌头碱、乌头碱、次乌头碱)”和“总葸醌—游离蒽醌—结合葸醌”多层次量化评价体系。在不同配伍比例和煎煮时间条件下,研究附子和大黄饮片的配伍以及附子各组分(总生物碱、总多糖)与大黄各个组分(总葸醌、鞣质、总多糖)配伍后乌头类生物碱成分和蒽醌类成分的含量变化,确立不同配伍关系中有效成分的变化规律。结果:1.药材配伍方面,大黄用量增加能显著增加附子总生物碱含量;附子大黄药材1:0.5配伍降低双酯型生物碱的含量的作用最大。煎煮时间有利于升高总生物碱含量,也能促进双酯型生物碱的水解。2.附子大黄1:1配伍可升高结合蒽醌的含量。煎煮时间减少,结合葸醌含量有所升高。3.组分配伍方面,附子总生物碱1:1配伍大黄总葸醌能增加附子总生物碱的含量,大黄多糖与附子总生物碱配伍也能增加总生物碱含量,鞣质反之。随煎煮时间的延长,双酯型生物碱含量呈下降趋势,其中大黄多糖、鞣质是减低双酯型生物碱的关键因素,用量越大,下降越明显。4.附子总碱是影响大黄葸醌类成分的的关键因素,附子总碱的增加能降低总葸醌类含量,对结合葸醌的含量影响较小。与附子多糖配伍能降低总蒽醌,而使结合蒽醌含量上升。煎煮时间对总蒽醌影响不大,但对结合蒽醌影响较大,以30～60分钟内效果最佳,超过30分钟均有较大幅度下降。结论:附子热性成分——乌头类生物碱对大黄寒性泻下成分——大黄总蒽醌和结合蒽醌具有双向调控作用。附子多糖、比例、煎煮时间是附子附子调控大黄寒性泻下成分含量的关键因素。更多还原

【Abstract】 Objective:Study various factors(complication proportion,decoction time,ahead decoction or later decoction) in RALP and RRR traditional complication how to affect index component content.Attempt to initially uncover the key factors and material basis of herbs properties regulation about RALP&RRR cold-warm complication.Provide scientic explanation about RALP&RRR tradional complication theory which can change cold-purging into warm-purging function.Methods:Apply modern analysis technoledges such as UV,HPLC,TLCS etc.to study the complication of Radix Aconite Lateralis Prepapata(RALP) and Radix et Rhiaoma Rhei(RRR). Taking total alkanoids which is consided the warm property component of RALP and total anthraquinone which is consided the cold property componetnt of RRR as index to establish a polylevel quantitative evaluation system.The system which include total alkanoids-ester alkanoids-diterpenoid alkanoids of RALP and total anthraquinone-free anthraquinone-bind anthraquinone of RRR can help study index component content under different complication proportion and decoction time conditions.The study is carried out at 2 levels:herbs complication and active extracts complications.Determining the content of RALP alkanoids component and RRR anthraquinone component to affirm the active constitutions variation rules at different complication relationship.Results:1.At herbs complication level,the increasing of RRR can promote RALP total alkanoids content;when RALP complication RRR at 1:0.5,it can decrease diterpernoid alkanoid at largest extent.Decoction time increase can elevate total alkanoid and decrease diterpernoid alkanoids. 2.When RALP complication RRR at 1:1 and decrease decoction time can both promote bind anthraquinone content.3.At active extracts complication,when RALP total alkanoids complicates RRR total anthraquinone at 1:1 can increase total alkanoids content.RRR polysaccharide can also increase total alkanoids content.On the contrary,tanni can decrease toal alkanoids content.With the decoction time prolong diterpernoid alkanoids decreases,RRR polysaccharide,tanni both can cause diterpernoid alkanoids decrease.4.The RALP total alkanoids increase can cause total anthraquinone decrease,but it has slight effect on bind anthraquinone.RALP polysaccharide has a similar effect upon anthraquinone.Decoction time has slight effect on total anthraquinone but can heavilily affect bind anthraquinone,when decoction time exceed 30 minutes the bind anthraquinone decrease obviously.Conclusion:RALP warm constitution—alkanoids can duplex regulate RRR cold purging constitution—total anthraquinone and bind anthraquinone.RALP polysaccharide,complication proportion and decoction time is the key factor for RALP regulating RRR cold purging constitution.更多还原