【作者】 任贺庄；

【导师】 吕学诜； 胡林； 尤其敏； 罗佳滨；

【作者基本信息】 佳木斯大学 ， 遗传学， 2008， 硕士

【摘要】 目的:建立一种基于PCR扩增结合封闭式核酸快速检测装置(内含核酸检测试纸条)新的基因分型方法,对四种常见人乳头瘤病毒(HPV)进行快速诊断与分型,以区分生殖道感染中最常见的低危性HPV6,11和高危性HPV16,18基因型,为临床常见HPV诊断和分型提供一种快速、准确、无需昂贵仪器、;廉价的诊断试剂。方法与结果: 137例临床HPV16-18和HPV6-11阳性的标本通过DNA提取,PCR扩增,封闭式核酸快速检测装置的检测与分型,将其产物部分测序和与广州达安基因公司荧光定量PCR结果做比较,其符合率达到97%以上。结论:结果表明该方法能够对四种最常见人乳头瘤病毒(HPV)进行快速诊断与分型,该方法简单、快速、准确、经济,不需要高级的检测设备,对宫颈棉拭子样本和瘤体样本均适用。该方法适合于各级医院尤其是中小型医院对人乳头瘤病毒(HPV)进行快速诊断与分型,具有重要的临床推广价值。更多还原

【Abstract】 Objective: Develops a novel genotyping method for detecting the four types of human papillimovirus (HPV6, HPV11, HPV16, HPV18) which based on the polymerase chain reaction (PCR) and cross-contamination-proof (XCP) nucleic acid detection device.Methods and Results: After the sample preparation and PCR amplification, the XCP nucleic acid detection device was used for HPV detection and genotyping .Compared the results of 137 clinical HPV positive samples with real time PCR, the coordination rate was beyond 97 percent.Conclusion: The results showed that the new genotyping method can detect HPV genotypes and is simpler, faster, more accurate, cheaper, no expensive-equipment needed and cross-contamination-proof comparing with other method. It could be used in the small hospital and the clinics in resource-limited area and have an important promote value.更多还原