【作者】 王鸾；

【导师】 王振宇； 卢卫红；

【作者基本信息】 哈尔滨工业大学 ， 生物化工， 2007， 硕士

【摘要】 通过超声波-超临界CO2联用法提取大果沙棘果油,对提取工艺进行研究,确定了优化的提取工艺条件为:超声强度128w,超声时间40min,料液比1:12条件下对大果沙棘果渣进行超声波辅助提油,之后用萃取压力24MPa,萃取温度33℃,萃取时间75min,分离Ⅰ压力10MPa、分离Ⅰ温度35℃、分离Ⅱ压力8MPa、分离Ⅱ温度38℃的超临界萃取设备继续提油,所提的油得率为9.24%,亚油酸得率为2.44%。用超声波-超临界CO2联用法提取大果沙棘果油是一种创新的提取方法,实验表明这种联用法具有实际意义。用尿素包合法和硝酸银硅胶柱层析法纯化大果沙棘果中亚油酸,通过响应面分析法优化尿素包合法纯化亚油酸的工艺参数,确定的纯化条件为:尿素:FFA=2.4:1;反应温度为66℃;包合时间为11.7h,纯化得到的亚油酸含量为57.66%,亚油酸得率为5.88%。再用AgNO3:硅胶=1:10,PUFA:吸附剂=1:25的柱层析条件进一步纯化亚油酸,经气相色谱分析确定可纯化得到的最高亚油酸含量为82.53%,亚油酸回收率为82.63%。实验结果表明,尿素包合法和硝酸银-硅胶柱层析法是有效的纯化亚油酸的方法。通过药效学试验研究纯化得到的大果沙棘果亚油酸对小鼠的降血脂活性,结果为大果沙棘果亚油酸对高血脂模型小鼠的TC和TG水平具有明显的降低作用(p<0.05),对HDL-C水平有明显升高作用(p<0.02),证明大果沙棘果亚油酸具有降血脂功能。更多还原

【Abstract】 Extrated oil from fruit residue of Hippophae rhamneides by ultrasonic-SFE. The optimized extraction condition: in the condition of ultrasonic intensity 128w; ultrasonic time, 40min; the ratio between material and solvent is 1:12 using ultrasonic extract fruit oil, then pouring in the extraction department of SFE, in the condition of extraction pressure, 24MPa; extration temperature, 33℃,extraction time, 75min; separationⅠpressure, 10MPa; separationⅠtempatrature, 26℃; separationⅡpressure, 8MPa; separationⅡtemparature, 38℃, at this condition, the yield of oil is 9.24% and the yield of LA is 2.44%. The extract method of ultrasonic-SFE is an innovative method. The extraction experiment result show the method is practical meaning. Urea adduct method and silver-nitrite silica gel columniation chromatogram was adopted to pruifity LA of Hippophae rhbmneides. Study the purifitying technics parameter of urea adduct method by using response face analysis to determine the optimed purifity condition: FFA:urea= 2.4:1; reaction temperature 66℃; adduct time 11.7h. At the condition, the content of LA is 57.66% and the yield of LA is 5.88%. Using silver silica gel of silver-nitrite: silica gel =1:10 as the sorbent, in the condition of sample: sorbent=1:25 using columniation chromatogram further purify LA. By GC analyse the purifying result, the content of LA is 82.53% and the reclaim rate of LA is 82.63%. The experiment result show that Urea adduct method and silver silica gel of silver-nitrite method effectively purify LA. The lowering hyterlipemia lever’s activity was studied by medical trial. LA can significantly fall the level of TC and TG (p<0.05), can significantly hoist the level of HDL-C (p<0.02) .The result show that LA has the function of lowering hyterlipemia.更多还原