【作者】 路选；

【导师】 蔡军；

【作者基本信息】 南昌大学 ， 外科学， 2007， 硕士

【摘要】 目的1.研究端粒酶逆转录酶(hTERT)和Bcl-2基因在胆管癌患者胆汁中的表达及相互关系。2.探讨检测胆汁中hTERT和Bcl-2基因对胆管癌的诊断价值。方法1.对照组(胆总管结石患者胆汁标本20份),试验组(胆管癌患者胆汁标本20份),2.采用半定量逆转录一聚合酶链反应(RT-PCR)方法检测标本中hTERT和Bcl-2基因。结果1.20例胆管癌患者胆汁中hTERT基因表达阳性14例(14/20),20例胆总管结石患者胆汁中hTERT基因无表达,两组间有统计学差异(p＜0.05);2.20例胆管癌患者胆汁中Bcl-2基因表达阳性11例(11/20),20例胆总管结石患者胆汁中Bcl-2基因的阳性1例(1/20),两组间有统计学差异(p＜0.05);3.10例胆管癌手术者中的6例有转移的胆管癌胆汁hTERT基因表达阳性6例(6/6)显著高于无转移的胆管癌胆汁hTERT基因表达(1/4)(p＜0.05)。10例胆管癌手术者中的4例高、中分化胆管癌中,其胆汁中Bcl-2基因阳性4例(4/4),低分化胆管癌患者胆汁中Bcl-2表达(2/6),两组无显著差异。4.胆管癌患者胆汁中hTERT和Bcl-2基因表达无相关性。结论1.胆管癌患者胆汁hTERT基因与Bcl-2基因的表达均明显高于胆总管结石者(p＜0.05)。2.端粒酶和Bcl-2在胆管癌胆汁中的表达无相关性。3.用RT-PCR方法检测胆汁中hTERT和Bcl-2基因的表达对胆管癌的诊断有参考意义。更多还原

【Abstract】 Objective:1.To study the expression of telomerase reverse transcriptase(hTERT) and Bcl-2 in bile of patients with cholangiocarcinoma and the relationship between them.2.To discuss the diagnostic value of the determination of the expression of hTERT and Bcl-2 in bile for cholangiocarcinoma.Methods:1.The control group(20 bile samples of patients with choledocholith).The trial group(20 bile samples of patients with cholangiocarcinoma).2.Expression of hTERT and Bcl-2 in bile were confirmed by reverse transcriptase polymerase chain reaction(RT-PCR)Results:1.hTERT were expressed in 14 out of the 20 bile samples of patients with cholangiocarcinoma(14/20),but no expression was found in 20 bile samples of patients with choledocholith,which was significantly different between the two groups(p＜0.05);2.Bcl-2 were expressed in 11 out of the 20 bile samples of patients with cholangiocarcinoma(11/20),but in only 1 out of the 20 bile samples of patients with choledocholith(1/20)、which was significantly different between the two groups(p＜0.05);3.In 10 patients with cholangiocarcinoma surgery, hTERT were expressed in 6 out of 6 bile samples of patients with metastatic cholangiocarcinoma(6/6),which was significantly higher than that of patients with non-metastatic cholangiocarcinoma(1/4)(p＜0.05).In 10 patients with cholangiocarcinoma surgery,Bcl-2 were expressed in 4 out of 4 bile samples of patients with highly or moderately differentiated cholangiocarcinoma(4/4),Bcl-2 were expressed in 2 out of 6 bile samples of patients with poorly differentiated cholangiocarcinoma(2/6),there no significant difference between them.4.The expression of hTERT and Bcl-2 in bile of patients with cholangiocarcinoma had no correlation.Conclusion:1.The expression of hTERT and Bcl-2 in bile of patients with cholangiocarcinoma were significantly higher than those of patients with choledocholith(p＜0.05).2.The expression of hTERT and Bcl-2 in bile of patients with cholangiocarcinoma had no correlation.3.Determination of the expression of hTERT and Bcl-2 in bile by RT-PCR has diagnostic value for cholangiocarcinoma.更多还原