【作者】 左云海；

【导师】 路名芝；

【作者基本信息】 南昌大学 ， 病理学与病理生理学， 2008， 硕士

【摘要】 目的:观察弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma DLBCL)中CARD11、NF-κB和Bcl-2表达情况,探讨它们在弥漫性大B细胞淋巴瘤中不同免疫分型中表达的意义及相互关系。方法:选取弥漫性大B细胞淋巴瘤60例和反应性增生的淋巴结20例,本组病例手术前均未经放疗和化疗。采用组织芯片技术和免疫组织化学方法对弥漫性大B细胞淋巴瘤进行免疫分型并检测弥漫性大B细胞淋巴瘤免疫学类型GCB和ABC型中CARD11、NF-κB和Bcl-2表达情况。结果:①60例弥漫性大B细胞淋巴瘤CD10、Bcl-6、MUM1和CD138的阳性表达率分别为30.0%、58.3%、58.3%、1.7%。②免疫分型: 60例弥漫性大B细胞淋巴瘤中免疫类型GCB25例,ABC35例。GCB25例中CD10(+)/Bcl-6(+)14例,CD10(-)/Bcl-6(+)1例; ABC35例中CD10(-)/bcl-6(-)21例, CD10(-)/Bcl-6(+)14例,MUM1(+)/CD138 ( - ) 13例, MUM1(+)/CD138(+)1例。③60例弥漫性大B细胞淋巴瘤中免疫类型中CARD11、Bcl-2和NF-κB表达情况:免疫类型GCB中CARD11的阳性率为24.0%,ABC中CARD11的阳性率为57.4%,二者之间P<0.05;GCB中Bcl-2的阳性率为32.0%,ABC中Bcl-2的阳性率为62.9%,二者之间P<0.05;GCB中NF-κB的阳性率为28.0%,ABC中NF-κB的阳性率为68.6%,二者之间P<0.05。CARD11阳性弥漫性大B细胞淋巴瘤中Bcl-2阳性率为80.8%, CARD11阴性弥漫性大B细胞淋巴瘤中Bcl-2阳性率为26.5%,二者之间P<0.05;NF-κB阳性弥漫性大B细胞淋巴瘤中Bcl-2阳性率为58.1%,NF-κB阴性弥漫性大B细胞淋巴瘤中Bcl-2阳性率为41.0%,二者之间P<0.05;CARD11阳性弥漫性大B细胞淋巴瘤中NF-κB阳性率为76.9%,CARD11阴性弥漫性大B细胞淋巴瘤中NF-κB阳性率为32.4%,二者之间P<0.05;结论:①CARD11、Bcl-2、NF-κB高表达与弥漫性大B细胞淋巴瘤的ABC类型相关。②CARD11、Bcl-2、NF-κB的表达相互之间存在正相关。更多还原

【Abstract】 Objective: To study the expression and significance of CARD11、Bcl-2、NF-κB in the common immunology types of diffuse large B-cell lymphoma and explore the correlativity during them.Materials and Methods: 60 specimens of diffuse large B-cell lymphoma patients and 20 specimens of reactive hyperplasia lymph node were selected, CARD11,Bcl-2 and NF-κB were studied by using tissue chip technique and the MaxVision immunohistochemical methods.Results:①The positive rates of CD10,Bcl-6,MUM1 and CD138 in 60 specimens of diffuse large B-cell lymphoma patients were 30.0%、58.3% ,58.3% and 1.7% respectively.②In 60 specimens of diffuse large B-cell lymphoma patients. immunology type: GCB 25 cases, ABC35 cases. 14 cases had the profile CD10(+)/bcl-6(+) and 11 cases had the profileCD10(-)/Bcl-6(+) , 21 cases had the profile CD10(-)/Bcl-6(-), 13 cases had the profile CD10(-) /bcl-6(+)/MUM1(+)/CD138(-) and 1 case had the profile CD10(-)/Bcl-6(+)/MUM1 (+) /CD138(+).③In 60 specimens of diffuse large B-cell lymphoma patients. Immunology type: GCB 25 cases, ABC 35 cases. GCB 25 cases, the positive rates of CARD 11was 24.0% ,ABC 35 cases, the positive rates of CARD11 was57.4%, there being significant difference between the two groups(P<0.05); GCB 25 cases, the positive rates of Bcl-2 was32.0% ,ABC35 cases, the positive rates of Bcl-2 was 62.9%, there being significant difference between the two groups(P<0.05); GCB 25 cases, the positive rates of NF-κB was 28.0% ,ABC 35 cases, the positive rates of NF-κB was 68.6%, there being significant difference between the two groups(P<0.05); The positive rates of Bcl-2 in CARD11 positive and native specimens were 80.8%、26.5% respectively, there being significant difference between the two groups( P<0.05). The positive rates of Bcl-2 in NF-κB positive and native specimens were 58.1%、41.0% respectively, there being significant difference between the two groups( P<0.05). The positive rates of NF-κB in CARD11 positive and native specimens were 76.9%、32.4% respectively, there being significant difference between the two groups( P<0.05).Conclusion: 1、The high expression of CARD11、NF-κB and bcl-2 were related to ABC immunology type of diffuse large B-cell lymphoma ; 2、There is significant positive correlation between CARD11 and Bcl-2, CARD11 and NF-κB, Bcl-2 and NF-κB , respectively.更多还原