【作者】 尹华；

【导师】 李美华；

【作者基本信息】 南昌大学 ， 外科学， 2008， 硕士

【摘要】 目的牙釉质细胞型颅咽管瘤发生的分子机制目前仍不清楚。本研究拟建立牙釉质细胞型颅咽管瘤的基因表达谱,筛选差异表达基因,进一步分析差异表达基因与牙釉质细胞型颅咽管瘤的关系。方法采用含有16450个Unigene的cDNA微阵列分析牙釉质细胞型颅咽管瘤组织与正常垂体柄组织表达谱的变化,应用荧光定量PCR技术验证cDNA微阵列检测出差异基因的可靠性,分析牙釉质细胞型颅咽管瘤组织相关基因的差异表达。结果二倍以上的差异表达基因453条,表达增加195条,表达减少258条。随机选择CGA、HBG2、ADAMDEC1、SCAPER和TRPM2基因做RT-PCR验证,发现ADAMDEC1、CGA、HBG2及TRPM2基因在牙釉质型颅咽管瘤组织中存在表达异常。结论初步筛选出牙釉质型颅咽管瘤异常表达基因。更多还原

【Abstract】 Objective:The molecular mechanism of the pathogenesis of adamantinomatous craniopharyngiomas is unclear. The study was designed to establish the gene expression profile of adamantinomatous craniopharyngiomas and investigate the diferential gene expression related to adamantinomatous craniopharyngiomas.Methods:The changes of gene expression profile between adamantinomatous craniopharyngiomas and normal tissue of stalk hypophysial were analyzed by cDNA microarray which represented approximately 16450 Unigene that would be tested in the assay.RT-PCR technology were employed to validate the authenticity of cDNA microarray.Results:453 genes that were differentially expressed in tumors and normal tissues were identified;Of which 195 genes were up—regulated and 258 were down—regulated.ADAMDEC1,CGA,HBG2,SCAPER and TRPM2 genes were selected randomly and were testified by Real-Time quantitative PCR.We found that ADAMDEC1,CGA,HBG2 and TRPM2 genes were differential expression gene.Conclusions : several genes are invovled in the pathogenesis of adamantinomatous craniopharyngiomas,however,their relation to the formation of adamantinomatous craniopharyngiomas needs to be declared.更多还原