【作者】 郭昌平；

【导师】 刘通；

【作者基本信息】 南昌大学 ， 外科学， 2008， 硕士

【摘要】 目的:研究S100A4基因在膀胱癌组织与正常膀胱组织中的差异表达,结合病理及临床资料明确S100A4基因表达水平与膀胱癌分化转移的关系,为膀胱癌侵袭转移的诊治提供依据。方法:取2003年4月至2007年4月诊断为膀胱癌病人并行手术切除的组织65例,包括不同的病理分型和临床分期。运用免疫组织化学S-P方法检测S l 00A4蛋白在膀胱癌中的表达情况并进行统计学分析。结果:在膀胱癌组织中S100A4均为高表达,统一计学分析结果均具有显著性差异,P<0. 05。在不同组织学分级中S100A4在底分化组织中的表达水平明显高于高分化组织,P<0. 05,二者有显著差异。在不同的手术一病理分期中S100A4在III , IV期组织中的表达明显高于I、II期组织中的表达,二者差异有显著性,P<0. 05。结论1.膀胱癌组织中S 100A4基因表达明显高于癌旁正常组织,可望作为膀胱癌的病情发展及指导临床治疗的标记物之一。2.膀胱癌低分化组病人S100A4基因阳性表达高于膀胱细胞癌高分化组病人,故S100A4基因表达与膀胱癌分化有关,癌组织S100A4测定有助于判定肿瘤的预后。3. S100A4基因差异表达与患者年龄、性别均无明显相关性。更多还原

【Abstract】 Objective: To investigate the differential expression of S100A4 in both bladder cancer and common bladder. To study the relationship between S100A4 expression level and differentiation and metastasis of bladder cancer combined clinical data and pathology together.Methods: the documents of 65 cases were taken from the patients undergone lobotomy of bladder because of bladder cancer between April 2003 and April 2007,including various patho-grouping and clinical stage.Immunohistochemical staining (S-P method) for S100A4 protein in bladder cancer was performed and analyzed by statistics. To identify relation between the expression of S100A4 protein Results: S100A4 in bladder cancer all had higher expression than they were in normal bladder (P<0. 05).The expression level of S100A4 in less-differentiated group was higher than that of well-differentiated group. Moreover, the expression level in stage III、IV was higher than that of stage I、II .Conclusions:1. Expression of S100A4 in bladder cancer tissues was higher than normal bladder tissues besides carcinoma. S100A4 may be one of markers on assessment of patient’s condition developing and clinical treatment instruction in bladder cancer.2. Expression of S100A4 in low-differentiation tissues was higher than in high-differentiation tissues .S100A4 was correlated to bladder cancer differentiation and may play an important role in prognosis of bladder cancer.3 There was no relationship between differential expression of S100A4 and age or sex of patients.更多还原