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紫癜圣愈散对CITP模型小鼠外周血共刺激分子CD86影响的实验研究
The Study of Zidian Shengyu Pulvis Affects on Costimulatory Molecules CD86 in Model Mice with CITP
【作者】 武君；
【导师】 秦克力；
【作者基本信息】 黑龙江中医药大学 ， 中西医结合临床， 2008， 硕士
【摘要】 目的:通过观察应用紫癜圣愈散后CITP模型小鼠外周血中血小板计数及共刺激分子CD86水平的变化,初步探讨共刺激分子CD86与CITP发病机制的关系及紫癜圣愈散对CITP的作用机理。方法:采用注射豚鼠抗小鼠血小板血清(APS)的免疫造模方法,建立BALB/c小鼠CITP模型;将BALB/c小鼠60只随机分为6组:正常组、模型组、激素对照组、紫癜圣愈散低、中、高剂量组;应用紫癜圣愈散对模型小鼠进行实验研究,进行实验观察:(1)全自动血细胞计数仪检测CITP模型小鼠血小板计数;(2)酶联免疫吸附法(enzyme-linkedimmunosorbent assay,ELISA)测定CITP模型小鼠外周血CD86的水平;(3)观察各组小鼠造模及用药前后毛色、活动、大便、反应、出血点和瘀斑等一般情况的变化。结果:①造模后小鼠的血小板减少,模型对照组与正常对照组相比,有显著差异(P＜0.05),模型对照组与正常对照组相比CD86水平明显升高,有显著差异(P＜0.05),初步证实了模型复制成功;②紫癜圣愈散低、中、高剂量组、激素组均能提高模型小鼠PLT计数,激素组、紫癜圣愈散中、高剂量组两两比较(P＞0.05)无显著性差异;紫癜圣愈散低剂量组与激素组比较(P＜0.05)有显著性差异;③紫癜圣愈散低、中、高剂量组、激素组均能降低模型小鼠CD86水平,激素组、紫癜圣愈散中、高剂量组两两比较(P＞0.05)无显著性差异;紫癜圣愈散低剂量组与激素组比较(P＜0.05),有显著性差异;④各治疗组的小鼠一般状态均较模型对照组好,进食量、活动量增加,出血现象减轻。结论:紫癜圣愈散能升高模型小鼠外周血小板数,降低CITP模型小鼠外周血中CD86水平,对BALB/c小鼠CITP动物模型具有较好的治疗效果。
【Abstract】 Objective:To investigate the mechanism by which Zidian Shengyu Pulvis(ZSP)affects the development of CITP through observing changes of PLT number and the peripheral blood expression of CD86 in CITP model mice.Methods:1.The mice were divided into six groups:normal,model,hormone control,low dose of ZSP,middle dose of ZSP,and high dose of ZSP.2.The chronic ITP model was established by intraperitoneally injection of anti-BALB/c platelet serum(APS).3.The platelet number in mice was calculated by an automatic blood cell counter.The peripheral blood expression of CD86 in mice was measured by ELISA.The mice in treatment groups were gavaged with ZSP for 14 consecutive days starting 8 hours agter the 1st abdominal cavity injection of APS.Results:The PLT number in mice of the model group was lower than that in normal group,and the expression of CD86 in mice of the model group was statistically higher than that in the normal group(P＜0.05).Compared with the model group,mice in all the treatment groups showed statistically higher PLT number and lower CD86 expression levels(P＜0.05).No significant difference was observed among the middle dose of ZSP,high dose of ZSP and the hormone group.Conclusion:CD86 was expressed in peripheral blood at higher level in the ITP model mice.ZSP has functions of lowering peripheral blood expression of CD86 and increasing PLT number.It plays a role in the treatment of ITP by regulating immunological functions to reduce PLT destruction.