【作者】 张雪梅；

【导师】 牛铭钢；

【作者基本信息】 黑龙江中医药大学 ， 中医妇科学， 2008， 硕士

【摘要】 目的:观察妊娠期高血压疾病患者血清中Th1型细胞因子(TNF-α)和Th2型细胞因子(IL-10)水平,以及Th1/Th2(TNF-α/IL-10)比值的变化,探讨上述指标在妊娠期高血压疾病发病中的意义。方法:采用ABC-ELISA法测定64例妊娠期高血压疾病患者(其中22例妊娠期高血压组;24例子痫前期组;18例子痫组);25例正常对照组血清中TNF-α和IL-10的水平,并计算Th1/Th2(TNF-α/IL-10)比值;比较各组间细胞因子水平及Th1/Th2比值的差异。结果:①与对照组相比较,妊娠高血压组TNF-α水平无显著差异,(p＞0.05);子痫前期组及子痫组TNF-α水平均有极显著差异,(p＜0.001),且随病情加重而呈逐渐增加趋势。②妊娠高血压疾病患者血清中IL-10水平随病情加重而呈下降趋势,其中妊娠高血压组无显著差异(p＞0.05);子痫前期组及子痫组IL-10水平均有极显著差异(p＜0.001)。③与正常对照组相比较,妊娠高血压组TNF-α/IL-10比值无显著差异,(p＞0.05);子痫前期组及子痫组TNF-α/IL-10比值均有显著差异,(p＜0.001)。结论:妊娠期高血压疾病患者Th1型细胞因子TNF-α表达增加,而Th2型细胞因子IL-10表达下降,Th1/Th2比值显著增高。说明妊娠期高血压疾病患者存在着免疫失衡,细胞因子TNF-α和IL-10水平以及TNF-α/IL-10的平衡改变是其重要的病理生理变化之一,可能与妊娠期高血压疾病的发生、发展有关。更多还原

【Abstract】 Objective: To investigate the changes in serum levels of Th1-type cytokines (TNF-α) and Th2-type cytokines (IL-10) and the ratios of Th1/Th2 ( TNF-α/IL-10) in gestational hypertension. Methods: IL-10 and TNF-αwere determined with ABC-ELISA in serum samples from 22 women with gestational hypertension, 24 women with preeclampsia, 18 women with eclampsia and 25 normal term pregnant women. The Th1/Th2 ratios were calculated accordingly. Results: There were no significant differences in serum levels of IL-10 and TNF-α(P> 0. 05 for all) among normal pregnancy and gestational hypertension . There were significant differences in serum levels of IL-10 and TNF-α( p < 0.001 for all) among preeclampsia and eclampsia. The ratio of serum TNF-α/ IL-10 was no significantly higher among normal pregnancy and gestational hypertension (P>0.05), and the ratio of TNF-α/IL-10 significantly higher in patients with preeclampsia and eclampsia than that in either controls or gestational hypertension (P<0.001) .Conclusion: Gestational hypertension patients Th1-type cytokines TNF-αexpression increased, while Th2 cytokine IL-10 expression decline, Th1/Th2 ratio was significantly higher. Th1 / Th2 cytokines to the balance of Th1 migration, that is, enhance the functions of Th1 cells, decrease the functions of Th2 cells. Alterations of serum cytokine balance with predominance of Th1 immunity were observed in hypertensive disorder complicating pregnancy. These associations may offer insight into the pathogenesis of hypertensive disorder complicating pregnancy.更多还原