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炭材料对微生物生长影响的研究

Research about Growth Promotion Effects of Graphite on Microorganisms

【作者】 陈小利

【导师】 戴光泽;

【作者基本信息】 西南交通大学 , 材料学, 2008, 硕士

【摘要】 炭材料具有良好的生物兼容性的事实已被学界和业界普遍认同,并得到了非常广泛的应用。但是,炭材料生物亲和性的本质,却一直困扰着人们,各国学者也在努力探索。本论文着重探讨了炭材料对微生物生长过程的影响,它将有助于解释炭材料微生物亲和性的本质和生物膜法污水处理工艺中炭材料表面牢固、快速固着活性污泥现象的机理。为探寻炭材料对微生物生长的促进作用,本研究主要采用枯草芽孢杆菌和枯草芽孢杆菌黑色变种、啤酒酵母和白色念珠菌作为受体微生物,通过调整培养基成分和控制受体微生物的生长环境的方式:即对不同菌种分别添加化学抑制剂和石墨的方法,观察外界环境对受体微生物生长过程的影响情况,进而确定抑制剂和炭材料的存在对于微生物生长的影响程度。通过以上不同对比组中的不同菌种、不同培养条件等因素的对比,可以得到如下主要结论:1、受体微生物生长在部分受到抑制的条件下,石墨能显示出很强的生长促进作用,且这种促进作用能穿透玻璃传递给受体微生物,由此推测该作用不是化学作用,而可能是相互间的电磁作用;2、受体微生物生长在没有受到抑制和完全受到抑制两种条件下,石墨的作用不显着。

【Abstract】 The excellent biocompatibility of carbon materials are widely accepted and utilized by researchers and commercial applications currently. However, the mechanism of its excellent biocompatibility remains unknown for a long time. Many efforts are placed on the biocompatibility of carbon materials through international cooperation researches. The focuses of current research mainly concern the growth promotion effects of microorganism affected by graphite powders. The research is expected to be significant in elucidating the hypostasis about the biocompatibility of carbon materials, and explaining why the surface of carbon fibers could both quickly and firmly immobilize much of activated sludge in bio-film wastewater treatment.For verifying the growth-promotion effects of carbon materials on microorganism, the current research adopted Bacillus subtilis and Bacillus subtilis var. niger, Saccharomyces cerevisiae and canidia Albicans as receptor microorganisms, controlled the growth condition such as changing the component concentration of culture medium, adding chemical inhibitor and graphite powder, finally observed the growth process of microorganism, consequently confirmed the affection of inhibitors and graphite on these microorganisms.According to the comparison between different inhibitors and growth conditions mentioned above, it is concluded that:1. Graphite places a strong promotion effect on the growth of microorganisms when inhibited partially. This promotion effect can penetrate through the glass of the Petri dishes and transfer to receptor microorganisms, so it could be considered as electromagnetic effect rather than chemical factor.2. When the growth of receptor microorganism was inhibited absolutely or not at all, graphite can’t accelerate the growth speed evidently.

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