【作者】 郑安锡；

【导师】 霍钢；

【作者基本信息】 重庆医科大学 ， 外科学， 2008， 硕士

【摘要】 目的:探讨细胞周期相关蛋白cyclin E、cdk2和p27kip1在侵袭性垂体腺瘤中的表达,三者的表达有无相关性,以及它们的表达与垂体腺瘤呈侵袭性生长的生物学行为是否具有相关性,以期为侵袭性垂体腺瘤的诊断、临床治疗和预后判断提供帮助。方法:58例新鲜切除的垂体腺瘤标本每例分成三份,一份送病理科行常规病理检查,一份立即放入4%的多聚甲醛液中固定用于免疫组化检测,另一份立即放入-80℃冰箱冻存或立即提取蛋白用于免疫印迹检测。使用兔抗cyclin E、兔抗cdk2和兔抗p27kip1三种抗体,采用免疫组织化学法(Immunohistochemistry,IHC)和western印迹法(western blotting,WB)检测垂体腺瘤中cyclin E、cdk2和p27kip1蛋白的表达情况。结果:Cyclin E和cdk2在侵袭组中的表达均明显高于非侵袭组(P均<0.05),而p27kip1在侵袭组中的表达明显低于非侵袭组(P<0.05);cyclin E和cdk2在垂体腺瘤中的表达呈正相关(P<0.05),两者与p27kip1的表达均呈负相关(P均<0.05);cyclin E和cdk2的表达与垂体腺瘤的侵袭性均呈正相关(P均<0.05),p27kip1的表达与垂体腺瘤的侵袭性呈负相关(P<0.05)。结论: Cyclin E和cdk2的过度表达及p27kip1的低表达与侵袭性垂体腺瘤的发生、发展、增殖显著相关,三者可能成为判断垂体腺瘤的侵袭性和预后的指标。更多还原

【Abstract】 Objective: To study on the expressions of cell cycle associated protein cyclin E, cdk2 and p27kip1 in invasive pituitary adenomas, and to investigate whether there exist correlations among the expressions of the three proteins and between the expressions of the three proteins and the invasiveness of invasive pituitary adenomas.Methods: 58 cases of fresh resected pituitary adenomas were collected, each sample was divided into three parts, one for routine detection in the department of pathology; one for Immunohistochemistry by immediately dipping into 4% paraformaldehyde, and the last one for Western blotting analysis by storing in ultra low temperature refrigerator or extracting its proteins immediately. Immunohistochemical analysis and Western blotting analysis using anti-cyclin E, anti-cdk2 and anti-p27kip1 antibodies were performed to detect the expressions of cyclin E, cdk2 and p27kip1 in pituitary adenomas.Results: The expressions of cyclin E and cdk2 in invasive pituitary adenomas were both significantly higher than that in noninvasive pituitary adenomas (both P<0.05), but the expression of p27kip1 in invasive group was significantly lower than that in noninvasive group (P<0.05). There was positive correlation between the expression of cyclin E and cdk2 in pituitary adenomas (P<0.05), and negative correlation between the expression of the former two and p27kip1 (both P<0.05); there was positive correlation between the expression of cyclin E and cdk2 and the invasiveness of pituitary adenomas (both P<0.05), and negative correlation between the expression of p27kip1 and the invasiveness of pituitary adenomas (P<0.05).Conclusions: The overexpressions of cyclin E and cdk2 and the lower expression of p27kip1 were significantly associated with the invasiveness of pituitary adenomas, cyclin E, cdk2 and p27kip1 proteins may be valuable prognostic factors for pituitary adenoma.更多还原