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N~+束注入杏鲍菇耐高温菌株的选育研究

Study on Breeding of Pleurotus Eryngii with High Temperature Tolerance by N~+ Implantation

【作者】 周海燕

【导师】 张军; 曾宪贤;

【作者基本信息】 新疆大学 , 核技术及应用, 2008, 硕士

【摘要】 杏鲍菇是南欧、北非及中亚地区高山、草原、沙漠地带的一种品质优良的大型肉质伞菌,其质地脆嫩,菌肉肥厚,有杏仁的香味,口感极佳,是一种珍稀的药食皆宜的食用菌品种。杏鲍菇营养丰富、均衡,是一种高蛋白质,低脂肪的营养保健食品,含有18种氨基酸,长期食用可促进人体对脂类物质的消化吸收和胆固醇的溶解,对肿瘤也有一定的预防和抑制作用。它还含有利尿,健脾胃、助消化的酶类,具有强身、滋补、增强免疫力的功能。本研究首次应用低能氮离子注入进行杏鲍菇的育种研究,通过对注入后的杏鲍菇单核细胞悬液进行高温培养,筛选出一株耐高温菌株X0720;通过对耐高温菌株X0720和出发菌株XCK的液体发酵培养基和培养条件的优化研究,确定了最佳培养基和培养条件;同时测定了不同注入剂量的杏鲍菇突变株的三种胞外酶活,并进行了比较。

【Abstract】 Pleurotus eryngii is a large group of fleshy fungi which grows on alpine, grassland and desert of Southern Europe, Northern Africa and Central Asia. It is a kind of rare edible fungi used for medicine and food with a crumbly texture, amygdaline fragrance and pleasing taste. Pleurotus eryngii is nutritious and balanced healthy food with high protein, low fat and 18 amino acids. It can promote digestion and absorption of lipids and dissolution of cholesterol, and prevent tumour. It has a group of enzymes, some of which can promote the excretion of urine, and some of which are good for taste and acid digestion. It also has function of keeping fit and strengthening immune.This experiment is studied on Pleurotus eryngii breeding by ion beam implantation for the first time. N+ ion beam implanted Pleurotus eryngii mycelia were cultivated under high temperature. A high temperature tolerance strain was selected. The fermentation medium and some other factors of strains X0720 and XCK were optimized, and the optimum fermentation medium and cultivating condition were obtained. The three extracellular enzymes activity of the different mutant strains were measured.

  • 【网络出版投稿人】 新疆大学
  • 【网络出版年期】2009年 02期
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