【作者】 吴立根；

【导师】 陈盛；

【作者基本信息】 福建师范大学 ， 环境科学， 2008， 硕士

【摘要】 本文研究了产朊酵母菌利用烤鳗工序的四段废水产单细胞蛋白（简称SCP），选择其中产量最高的一段废水——蒲烧废水作为深入研究对象。接着研究产朊酵母菌利用蒲烧废水产单细胞蛋白的工艺条件，其结果为：接种量15％、发酵时间为48小时、温度在25℃、pH为5．0、硝酸钾浓度为5．04g／L、250毫升容量瓶装液量为40ml、磷酸氢二钠浓度为0．1g／L、无机盐ZnSO4·7H₂O浓度为0．1g／L等条件时，产朊酵母菌利用蒲烧废水（初始CODcr为1840mg／L）发酵SCP产量最高，可达1．3125g/L，其蛋白质含量达0．5053g／L。再研究了混合菌（产朊酵母菌和啤酒酵母菌）利用蒲烧废水产单细胞蛋白的工艺条件，结果表明：二种菌的复配比为1：1复配时SCP产量最大，二种混合菌产SCP的最佳发酵时间为24小时、250ml容量瓶装液量为50ml、温度在30℃、pH为5．0、硝酸铵浓度为1．33g／L、磷酸二氢钠浓度为0．2g／L等条件时，二种混合菌利用蒲烧废水（初始CODcr为4563mg／L）发酵SCP产量最高，可达1.726g／L，其蛋白质含量为0．6429g/L。研究结果表明：烤鳗废水可作为产单细胞蛋白的原料，单细胞蛋白是一种优质饲料蛋白源，这样不仅可以解决污水处理问题也可将废物资源化。更多还原

【Abstract】 The paper reported the Candida utilis which utilized four Eel Roasting working procedures wastewater to produce SCP （single cell protein）, and took the highest yield ofthe working procedure-the wastewater from Prepared Eel as the research object.Alsoincluded in this paper was a report of the optimum conditions that the Candida utilis utilized the wastewater from Prepared Eel to produce SCP. Results showed that:the amount of inoculation account for 15%、fermentation time is 48h、temperature 25℃、pH 5.0、KNO₃5.04g/L、liquid volume 40ml、Na₂HPO₄ 0.1g/L、and ZnSO₄·7H₂O 0.1g/L and so on, the Candida utilis utilized the wastewater from Prepared Eel（initial CODcr 1840mg/L）to ferment the highest yield SCP 1.3125g/L,protein content 0.5053 g/L.Furthermore,this paper reported the optimum conditions that the mixed bacteria（Candida utilis and Saccharomyces cerevisiae） utilized the wastewater from Prepared Eel to produce SCP. Results showed that: when combining proportion of two bacteria is 1:1, SCP is the highest yield,fermentation time is 241k liquid volume 50ml、temperature 30℃、pH 5.0、KNO₃1.33g/L、NaH₂PO₄ 0.2g/L and so on, the mixed bacteria utilized the wastewater from Prepared Eel （initial CODcr 4563mg/L） to ferment the highest yield SCP 1.726g/L,protein content 0.6429 g/L. Final results showed that: the wastewater from Eel Roasting can be used in producing SCP, and SCP is the high quality Dietary Protein Sources, which not only resolves the problem of sewage disposal,but also brings waste to the reutilization.更多还原