【作者】 朱晓东；

【导师】 吴文珊；

【作者基本信息】 福建师范大学 ， 生物化学与分子生物学， 2008， 硕士

【摘要】 爱玉子(Ficus awkeotsang Makino)为桑科榕属植物,雌雄异株,为我国特有。其瘦果制成的爱玉冻是种高贵的天然食品,人工栽培具有广阔的市场前景。在苗期及营养期很难从形态特征上来鉴别爱玉子的雌、雄以及品系,因而找到一种简单、迅速、准确的鉴别方法,是目前生产上亟待解决的难题。本实验采用SRAP分子标记技术,研究爱玉子性别鉴别、爱玉子优良品系鉴别及其遗传多样性,为爱玉子栽培和种质资源改良提供理论依据。通过对CTAB法进行改良,建立了适合爱玉子DNA提取的方法,获得了浓度和纯度都较高的基因组DNA。利用128个SRAP引物组合对爱玉子雌、雄DNA池进行扩增,从1228个扩增条带中找到一个雌性特异的标记Me5Em14-230,可用于爱玉子的早期性别鉴别。对福建省主栽的爱玉子品系(24个雌性品系,16个雄性品系)进行品系鉴别研究,结果表明:Me1Em8引物组合能够区分全部雄性品系,Me6Em6和Me3Em2双引物组合可以区分全部雌性品系;并在此基础上建立了雌、雄爱玉子各品系独特的数字化核酸指纹。进行Nei’s遗传距离的计算,并以UPGMA法聚类,结果表明,雌、雄爱玉子各品系之间存在着较为复杂的亲缘关系,具有较丰富的遗传多样性。更多还原

【Abstract】 Ficus awkeotsang Makino is a species of Moraceae in Ficus, and it is a dioecism and a unique woody vine growing in China. Its achenes can be utilized to produce jelly curd, which is an excellent food in the market. So, its artificial cultivation has broad market prospects. But it is hard to discriminate Ficus awkeotsang Makino’s sex and strain from its morphologic characteristics when they are not in blossom. A simple, rapid and accurate method of identification should be developed and applied in practical production. Sex and strains identification and the genetic diversity of Ficus awkeotsang Makino were studied in this work using SRAP(Sequence-related amplified polymorphism) molecular marker. The result could provide a theoretical basis for the cultivation and the improvement of germplasm resources in Ficus awkeotsang Makino.The genomic DNA of Ficus awkeotsang Makino with high concentration and purity could be obtained with an improved CTAB method, which was suitable for molecular analysis. 128 SRAP primer combinations were used for SRAP amplification and about 1228 fragments were obtained from male and female DNA pool. A marker of about 230bp linked to female was generated by Me5Em14 and was named Me5Em14-230. This marker could be used to identify different sex of Ficus awkeotsang Makino at the seedling stage. SRAP marker was imployed to identify different strains of Ficus awkeotsang Makino in this study, the result showed that a primer combination MelEm8 could identify the 16 male strains, and two primer combinations Me6Em6 and Me3Em2 could identify the 24 female strains. On the basis of the result, specific fingerprint of each strains was produced. More over, the result of Nei’s genetic distance and UPGMA cluster analysis indicated that the relationship between strains was very complicated, and the diversity of cultivated Ficus awkeotsang Makino was high.更多还原