【作者】 曹爱良；

【导师】 曹培国；

【作者基本信息】 中南大学 ， 内科学， 2007， 硕士

【摘要】 目的:通过检测胃癌组织中HGF、c-Met的表达和MLD值,探讨HGF、c-Met的表达和MLD值与临床病理因素之间的关系以及三者之间的相互关系,研究胃癌淋巴管转移的可能机制。方法:应用免疫组化方法检测52例胃癌病人癌组织中HGF、c-Met的表达,计数单克隆抗体D2-40标记的微淋巴管密度(MLD)。结果:1、胃癌组织中HGF及c-Met阳性表达均主要位于肿瘤细胞胞浆内,其表达阳性率分别为67.3%和78.8%。2、HGF的表达与组织学分级、浸润深度、临床分期、淋巴结转移显著相关(P＜0.05),与年龄、肿瘤大小无关(P＞0.05)。3、c-Met的表达与组织学分级及淋巴管转移密切相关,而与肿瘤大小、年龄、浸润深度、临床分期无关(P＞0.05)。4、胃癌中MLD值显著高于癌旁正常组织,与肿瘤浸润深度、临床分期无显著性相关(P＞0.05),而与组织学分型及淋巴结转移密切相关(P＜0.05)。5、52例胃癌组织中,HGF和c-Met共阳性表达者占31例,共阴性表达者7例,两者表达呈正相关(r=0.342,P＜0.05)。6、HGF表达阳性者的MLD值显著高于阴性者(P＜0.005)。结论:1.HGF及c-Met表达于大部分胃癌癌细胞胞浆内。2.HGF的高表达与胃癌组织学分级、浸润深度、临床分期及淋巴结转移密切相关;c-Met的高表达与胃癌的组织学分级及淋巴结转移密切相关。3.胃癌组织中MLD值显著高于癌旁正常组织,与肿瘤组织学分级及淋巴结转移密切相关。4.胃癌组织中MLD与HGF的表达密切相关,而与c-Met的表达无关。更多还原

【Abstract】 Objective:To detect the expressive levels of hepatocyte growth factor and c-Met and D2-40 in gastric carcinoma;To detect the MLD and its correlation with clinicopathological factors in gastric carcinoma;To investigate the possible mechanism of lymph node metastasis in gastric carcinoma.Methods:Immunohistochemistry method was used to detect the expression of HGF and c-Met and D2-40 in gastric cancer tissue and the normal tissues near the tumor borderline,with microlymphatic density (MLD)counted.Results(1).HGF and c-Met was expressed mainly in cytoplasm of gastric carcinoma cells.The expressive rate of HGF and c-Met in gastric cancer were 67.3%and 78.8%,respectively.(2).The expression of HGF had the positive correlation with histologic types,infiltration depth,clinical stage and lymph node metastasis(P＜0.05).No correlation was found in age,tumor diameter(P＞0.05).(3).The expression of c-Met had the positive correlation with histologic types and lymph node metastasis(p＜0.05).No correlation was found in age,tumor diameter,infiltration depth and clinical stage(P＞0.05).(4).The MLD in the gastric cancer was higher than that in the normal tissues near the tumor borderline(P＜0.05).The MLD had no correlation with infiltration depth and clinical stage(P＞0.05).The positive correlation was found in histologic types and lymph node metastasis(p＜0.05)(5).In 52 cases of gastric cancer,there were 31 cases where the expression of HGF and c-Met was positive simultaneously.The simultaneously negative was 7 cases.Correlation coefficient showed that HGF had a positive correlation with c-Met in gastric cancer(r=0.342, P＜0.05).(6).In gastric cancer,The MLD in the HGF-positive group was higher than that in the HGF-negative group(P＜0.05)Conclusion(1).HGF and c-Met are expressed in plasma in most gastric cancer cell.(2).The expression of HGF has correlation with histologic types, infiltration depth,clinical stage and lymph node metastasis in the gastric carcinoma.The expression of c-Met has correlation with histologic types and lymph node metastasis in the gastric carcinoma.(3).The MLD in the gastric cancer was higher than that in the normal tissues near the tumor borderline.The MLD has the positive correlation with histologic types and lymph node metastasis.(4).The MLD in the gastric cancer has correlation with the expression of HGF,but it has no correlation with the expression of c-Met.更多还原