【作者】 吕兰；

【导师】 张长杰；

【作者基本信息】 中南大学 ， 康复医学与理疗学， 2008， 硕士

【摘要】 目的:探讨坐骨神经损伤后TGF-β1表达变化及脉冲电磁场对TGF-β1表达的影响。方法;采用大鼠坐骨神经损伤动物模型,将52只SD大鼠随机分成正常组4只,治疗组、夹伤组,对照组每组16只,根据实验动物的手术时间和处死取材时间的不同,组内再随机分为术后1d,3d,7d,14d等4个时相观察点,各4只大鼠。夹伤组:夹伤右侧坐骨神经,予以空白电磁场治疗(磁场强度为0);治疗组:夹伤右侧坐骨神经,予以脉冲电磁场治疗;对照组:切开暴露坐骨神经后即缝合,不给予任何其他处理;正常组:不给与任何干预,保持同样饲养条件至试验结束。以坐骨神经功能指数(SFI)评定功能状况,HE染色观察组织学改变,免疫组化技术检测TGF-β1的表达,用计算机图像自动处理系统进行定量分析研究。结果:1.治疗组SFI值与损伤组相比无统计学意义(P＞0.05)。2:HE染色光镜下观察:治疗组神经变性程度较损伤组严重。3:TGF-β1在损伤神经中表达持续增高,在本次试验终点达到最高值,治疗组与损伤组相比,TGF-β1表达增高,术后1d,3d,7d,14d有统计学意义(P＜0.05)。结论:1 PEMFs对神经损伤早期功能的恢复无明显作用。2 PEMFs能加速损伤早期远侧神经段的Wallerian变性进程。3 TGF-β1在损伤神经中表达增高,在本次观察的时间点内呈逐渐升高趋势,PEMFs可使该上升曲线左移,说明PEMFs可促进周围神经损伤后TGF-β1的表达,提示PEMFs对周围神经的作用可能通过TGF-β1来实现。更多还原

【Abstract】 Objective: To observe the change of transforming growth factor beta expression after rat sciatic nerve crushed injury and TGF-β1 and the relationship of PEMFs and TGF-β1.Methods: 52 SD rats were divided into four groups: injury group A、PEMFs theropy group B、control group and nomal group D.The sciatic nerve were crushed with a special clamp.Group A were treated with PEMFs exposure and group B with sham explosure after the operation .Group C only exposed the sciatic nerve and group D was not treated any treatment .The histology and immunohistochemistry examination were performed respectively after 1 ,3 ,7,14 days.Result: 1 :The SFI show no difference comparing group A and group B(P> 0.05).2: The expression of TGF-β1 increased during the process of nerve regeneration, and reached the maximum 14days after nerve injury. There were distinct difference between group A and group C(P<0.05).3: The expression of TGF-β1 show distinct difference comparing group A with the group B on 1,3,7,14 days after the operation . PEMFs benefited the expression of TGF-β1 during nerve regeneration (P<0.05).Conclusion: The PEMFs didn’t have obvious effect on the recovery of sciatic nerve function . The PEMFs participate in the pathoiogic process of peripheral nerve damage . and PEMFs promotes the expression of TGF-β1 during scitic nerve regeneration.更多还原