【作者】 曾虎；

【导师】 聂晚频；

【作者基本信息】 中南大学 ， 外科学， 2008， 硕士

【摘要】 目的:血小板衍生生长因子（PDGF）是促进肝纤维化发展的重要因子之一。本课题研究脾切除对肝纤维化大鼠肝脏PDGF-B的表达和血清PDGF-BB水平的影响,从而探讨脾切除在肝纤维化治疗中的作用。方法:对大鼠皮下注射CCL₄,建立65例肝纤维化大鼠模型。于建模开始,肝纤维化期,及肝硬化期三组切除大鼠脾脏,并设立同期假手术组作为对照组。第一、二组及其对照组于建模后第10周,第三组及其对照组于建模后12周分别取大鼠肝脏和血液标本。用免疫组化SABC方法测定肝脏PDGF-B的表达,HE染色检测肝纤维化,应用双抗体夹心ELISA方法测定血清PDGF-BB水平。结果:（1）PDGF-B阳性物质主要位于阳性细胞的胞浆内,呈棕色颗粒。这些细胞分布在汇管区、纤维间隔、炎症区,少数在肝窦内。各切脾组PDGF-B阳性细胞A值（吸光度值）明显低于其对照组。（2）各切脾组血清中PDGF-BB含量明显低于其对照组。（3）根据HE染色,建模开始时切脾再行肝纤维化诱导,肝纤维化进程缓慢;建模第5周切脾后,肝脏病理状态比对照组明显缓解;肝硬化期切脾后,肝脏炎性改变和纤维化程度有一定减轻。结论:（1）脾脏切除能降低肝纤维化大鼠肝脏PDGF-B以及血清中PDGF-BB的水平,有助于缓解肝纤维化程度。（2）肝纤维化进程中越早切除脾脏对肝纤维化的延缓作用越大。更多还原

【Abstract】 Objectives:PDGF is one of important factors which can promote the development of hepatic fibrosis.To discuss the effects of splenectomy on Hepatic fibrosis,we tried to study the effects of splenenctomy on the expression of PDGF-B in the liver and the serum level of PDGF-BB of the rats with Hepatic fibrosis.Methods:By hypodermic injection CCL₄,we established 65 rat models with hepatic fibrosis,splenectomy were performed in three groups at different phases:before hypodermic injection CCL₄（A group）、five weeks after hypodermic injection CCL₄（B group）、tenth weeks hypodermic injection CCL₄（C group）.The control groups were established at the same time.then took samples of the livers and serum of the rats in different phases.The expressions of PDGF in the liver were detected by immunohistochemistry technique and the degree of hepatic fibrosis were detected by HE staining.The serum levels of PDGF-BB were analyzed by ELISA technique.Results:（1）PDGF-B was mainly distributed in the intracytoplasm of positive cells.them lay in portal areas、fiberseptation、inflammation areas,and a little in sinus hepaticus.Absorbance values of PDGF-B in the experimental group were significantly lower then The control groups（p＜0.05）.（2）The serum levels of PDGF-BB of the rats after splenectomy were significantly lower than the control groups.The serum levels of PDGF—BB of the rats among the experiment groups respectively were（pg/ml）: 37.856±5.893,199.425±17.089,340.718±116.894;However the numerus of the control groups respectively were（pg/ml）:1356.882±273.054,1095.150±100.956,1325.658±301.048（P＜0.05）. （3）We could detected the effects of splenectomy on hepaic fibrosis after HE and Masson’s staining:the progression of Hepatic fibrosis in A group is slowly;Hepatic pathologic state relieved significantly in B group; the inflammation and fibrosis is relieved in C group.Conclusion:（1）Splenectomy could degrade the levels of PDGF-B and The serum levels of PDGF-BB,which conduce relieve hepatic fibrosis.（2）The time of splenectomy is earlier,the effect of splenectomy on hepaic fibrosis is better.更多还原