【作者】 唐华燕；

【导师】 袁君；

【作者基本信息】 中南大学 ， 肿瘤学， 2008， 硕士

【摘要】 目的:探讨LaminB1租NMP238的表达与鼻咽癌放射敏感性及预后的关系。方法:1.收集52例经病理确诊为鼻咽低分化鳞癌患者的活检标本。52例患者治疗前经过相关检查均无远处转移,均予根治性放疗。52例患者根据放疗效果分为完全缓解组(CR)和部分缓解组(PR)。2.应用免疫组织化学SP法检测放疗前癌组织中LaminB1,MP238的表达水平。3.观察:(1)LaminB1和NMP238在鼻咽低分化鳞癌组织中的表达。(2)LaminB1和NMP238表达与临床分期的关系。(2)LaminB1和NMP238在鼻咽低分化鳞癌组织中表达的相关性。(3)LaminB1和NMP238表达与放射敏感性的关系。(4)LaminB1和NMP238表达与预后的关系。结果:1.LaminB1和NMP238在鼻咽低分化鳞癌组织中的阳性表率分别为79%、67%。2.LaminB1和NMP238表达强度在鼻咽癌各临床分期中有显著差异(P＜0.05)。3.在52例鼻咽癌组织中,pearson相关分析显示LaminB1,NMP238的表达呈显著正相关(C=0.446,P＜0.05)。4.52例鼻咽癌患者放疗结束后CR:36例,占69%;PR:16例,占31%;NC:0例;PD:0例。5.根据LaminB1表达强度分级,-～+++者鼻咽癌完全缓解率分别为81.8%(9/11)、79.3%(23/29)、37.5%(3/8)和25.0%(1/4),有显著差异(P＜0.05)。根据NMP238表达强度分级,-～+++者鼻咽癌完全缓解率分别为82.3%(14/17)、77.3%(17/22)、33.3%(3/9)和50%(2/4),有显著差异(P＜0.05)。6.52例鼻咽癌患者的五年生存率,Ⅰ～Ⅱ期五年生存率为84%,Ⅲ期五年生存率为53%,ⅣA期五年生存率为20%。7.根据LaminB1表达强度分级,-～+++者鼻咽癌五年生存率分别为72.7%(8/11)、72.4%(21/29)、25.0%(2/8)和25.0%(1/4),有显著差异(P＜0.05)。根据NMP238表达强度分级,-～+++者鼻咽癌五年生存率分别为82.3%(14/17)、68.2%(15/22)、22.2%(2/9)和25%(1/4),有显著差异(P＜0.05)。结论:1.LaminB1和NMP238表达强度在鼻咽癌各临床分期中有显著差异。2.LaminB1与NMP238在鼻咽低分化鳞癌组织中的表达具有显著正相关性。3.鼻咽癌组织中LaminB1与NMP238的表达强度与肿瘤放射敏感性以及预后有显著相关性。认为LaminB1与NMP238的表达状态可作为预测鼻咽癌放射敏感性及预后的指标。更多还原

【Abstract】 Objective:To investigate the relationship of the expression of LaminB1 and NMP238 in pre-radiotherapy tissue with the radiation sensitivity and prognosis in human nasopharyngeal carcinoma tissue.Methods:1.52 samples from patients with pathologically diagnosed as nasopharyngeal low-differentiation squamous cell carcinoma were collected.These patients had done some examinations before treatment and demonstrated that there was no distant metastasis.All cases had completed the radical radiotherapy.52 patients were classified into two groups according to the response to radiotherapy as complete response (CR)and partial response groups(PR).2.Immunohistochemical methods were performed to detect the expression levels of LaminB1 and NMP238 in pre-radiotherapy tissue.3.The positive expression rates of LaminB1 and NMP238 in nasopharyngeal carcinoma tissue were observed.The potential relations of expression of LaminB1 and NMP238 with clinic stage,radiation sensitivity and prognosis were also analyzed. Results:1.The positive expression rates of LaminB1 and NMP238 in nasopharyngeal low-differentiation squamous cell carcinoma were 79%、67%respectively.2.The expression intensities of LaminB1 and NMP238 in nasopharyngeal carcinoma significantly related with their clinical stages(P＜0.05).3.The expression of LaminB1 in nasopharyngeal carcinoma tissue was positively correlated with that of NMP238 by Pearson correlation analysis(C=0.446,P＜0.05).4.In 52 cases,only 36 cases(69%)were complete response,16 cases(31%)were partial response,while no change and progress disease were 0 and 0 respectively.5.According to the expression intensity of LaminB1(-～+++), the complete response rates were 81.8%(9/11)、79.3%(23/29)、37.5%(3/8) and 25.0%(1/4)respectively,and there was a significant correlation in them(P＜0.05).According to the expression intensity of NMP238(-～+ ++),the complete response rates were 82.3%(14/17)、77.3%(17/22)、33.3%(3/9)and 50%(2/4)respectively,and there was a significant correlation in them(P＜0.05).6.In 52 cases,the 5-year survival rates in stagesⅠ～Ⅱ、Ⅲ、ⅣA were 84%、53%、20%respectively. 7.According to the expression intensity of LaminB1(-～+++), the 5-year survival rates were72.7%(8/11)、72.4%(21/29)、25.0%(2/8)and 25.0%(1/4)respectively,and there was a significant correlation in them(P＜0.05).According to the expression intensity of NMP238(-～+ ++),the 5-year survival rates were 82.3%(14/17)、68.2%(15/22)、22.2%(2/9)and 25%(1/4)respectively,and there was a significant correlation in them(P＜0.05).Conclusions:1.There was a correlation between the expression intensities of LaminB 1 and NMP238 in nasopharyngeal carcinoma and their clinical stages.2.There was a co-relationship between the expression of LaminB1 and NMP238 in nasopharyngeal carcinoma.3.The expression intensities of LaminB1 and NMP238 significantly related with radiation sensitivity and prognosis.It suggests that the expression levels of LaminB1 and NMP238 can be as useful predictors for the radiosensitivity and prognosis in nasopharyngeal carcinoma.更多还原